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AB197452

Human Ig Isotyping 抗体 Array (8 Targets) - Quantitative

Human Ig Isotyping Antibody Array (8 Targets) - Quantitative

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ab197452 can be used for quantitative measurement of 8 Human immunoglobulins.

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Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

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Multiplex Protein Detection - Human Ig Isotyping Antibody Array (8 Targets) - Quantitative (AB197452)
  • Multiplex Protein Detection

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Multiplex Protein Detection - Human Ig Isotyping Antibody Array (8 Targets) - Quantitative (AB197452)

Standard Curves obtained with Abcam Human Ig Isotyping Antibody Array (8 Targets)- Quantitative (ab197452)

Multiplex Protein Detection - Human Ig Isotyping Antibody Array (8 Targets) - Quantitative (AB197452)
  • Multiplex Protein Detection

Supplier Data

Multiplex Protein Detection - Human Ig Isotyping Antibody Array (8 Targets) - Quantitative (AB197452)

Array Map for Human Ig Isotyping Antibody Array (8 Targets) - Quantitative (ab197452).

关键信息

检测类型

Quantitative

反应种属

Human

靶标

IGHG1

target

检测方法

Fluorescent

检测平台

Multiplex

样品类型

Serum

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产品详情

ab197452 can be used for quantitative measurement of 8 Human immunoglobulins. Suitable for blood serum.
Target names: IgA, IgD, IgE, IgM, IgG1, IgG2, IgG3, and IgG4.

Quantitative antibody arrays can be used to quantitate up to 40 cytokines with as little as 50 µL of sample. Arrays are available for 400 human and 200 mouse proteins. Each glass slide is spotted with 16 identical antibody arrays and is provided with a 16 well gasket to allow separate samples to be applied to each array. Each antibody is spotted in quadruplicate in each array. For reproducible quantitation, eight of the arrays are used with a cocktail of protein standards to produce a standard curve. The same 8 standard curve arrays can be used across multiple slides, allowing measurement of 22 experimental samples with 2 slides, 50 with 4 slides etc. For high through-put, 4 slides can be nested into a tray matching a standard microplate, allowing automated processing with a liquid handling workstation. Array processing can be completed within one working day (see workflow diagram below) and arrays can be analyzed with a wide number of laser glass slide array / gene microarray scanners. If you don’t have a suitable scanner then we recommend our membrane antibody arrays, which can be analyzed with any WB chemiluminescent reader.

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规格

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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
-20°C
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产品实验方案

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靶点信息

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
See full target information IGHG1
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websiteProtocolBooklet
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搭配使用产品

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RabMAb|Recombinant

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Assay Kits

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Secondary Antibodies

AB150191

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Human IgM mu chain (Alexa Fluor® 647) (AB150191)

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Assay Kits

AB108704

Human Anti-Adenovirus IgA ELISA Kit

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