人EGFR Phosphorylation抗体阵列-膜(17 Targets) (ab134005)
概述
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产品名称
人EGFR Phosphorylation抗体阵列-膜(17 Targets) -
样品类型
Tissue Extracts, Cell Lysate -
检测类型
Semi-quantitative -
种属反应性
与反应: Human -
产品概述
ab134005 is for simultaneous detection of the relative levels of 17 different Phosphorylated Human EGF Receptors. Suitable for cell and tissue lysates only.
Targets: EGFR (Tyr845), EGFR (Tyr992), EGFR (Tyr1045), EGFR (Tyr1068), EGFR (Tyr1086), EGFR (Tyr1148), EGFR (Tyr1173), EGFR (Ser1046/1047), EGFR (Ser1070), ErbB2 (Tyr877), ErbB2 (Tyr1112), ErbB2 (Tyr 1221/1222), ErbB2 (Tyr1248), ErbB2 (Thr686), ErbB2 (Ser1113), ErbB3 (Tyr1289), ErbB4 (Tyr1284)
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说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Multiplex Protein Detectionmore details
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 18µl 1 x 18µl 1 x 18µl 100X Phosphatase Inhibitor Cocktail Set I Concentrate 1 vial 2 vials 1 vial 1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml 20X Wash Buffer I 1 x 30ml 1 x 30ml 1 x 30ml 20X Wash Buffer II 1 x 30ml 1 x 30ml 1 x 30ml 2X Cell Lysis Buffer 1 x 5ml 1 x 5ml 1 x 5ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Cocktail of Biotin-Conjugated Anti-EGFR 2 vials 4 vials 1 vial Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml EGFR Phosphorylation Antibody Array Membranes 4 units 8 units 2 units Phosphatase Inhibitor Cocktail Set II 1 vial 2 vials 1 vial Protease Inhibitor Cocktail 1 vial 2 vials 1 vial -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134005于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Multiplex Protein Detection |
Use at an assay dependent concentration.
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说明 |
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Multiplex Protein Detection
Use at an assay dependent concentration. |
图片
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Multiplex Protein Detection - Human EGFR Phosphorylation Antibody Array - Membrane (17 Targets) (ab134005)
Human epidermoid carcinoma cell line, A431 cells that were 80-90% confluent were serum starved overnight, then exposed to 100 ng/ml EGF for 20 minutes at 37°C. Control cells were serum starved without the subsequent stimulation with EGF. Cell lysates were prepared according to the protocol. To use ab134005 Human EGFR Phosphorylation Antibody Array - Membrane, treated or untreated cell lysate was added into antibody array membrane. The antibody array membranes were washed and cocktail of biotinylated anti-EGFR was used to detect phosphorylated proteins on activated receptors. After incubation with HRP-Conjugated Streptavidin, the signals were visualized by chemiluminescence.
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Western blot analysis of extracts from 100 ng/ml hEGF treated A431 cells or untreated A431 cells. Phospho-EGFR (Tyr845) or Phospho-EGFR (Tyr1173) antibodies were used in this assay.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab134005 被引用在 6 文献中.
- Rosado-Galindo H & Domenech M Surface roughness modulates EGFR signaling and stemness of triple-negative breast cancer cells. Front Cell Dev Biol 11:1124250 (2023). PubMed: 36968199
- Yang KS et al. Propofol induces apoptosis and ameliorates 5‑fluorouracil resistance in OSCC cells by reducing the expression and secretion of amphiregulin. Mol Med Rep 25:N/A (2022). PubMed: 34859260
- Chen JC et al. Valproic acid-induced amphiregulin secretion confers resistance to temozolomide treatment in human glioma cells. BMC Cancer 19:756 (2019). PubMed: 31370819
- Fang H et al. EGFR inhibitor gefitinib regulates barrier function in human epidermal keratinocytes via the modulation of the expression of claudins. Int J Mol Med 43:1522-1530 (2019). PubMed: 30628660
- Lee PC et al. Targeting PKCd as a Therapeutic Strategy against Heterogeneous Mechanisms of EGFR Inhibitor Resistance in EGFR-Mutant Lung Cancer. Cancer Cell 34:954-969.e4 (2018). PubMed: 30537515
- Huang TW et al. The role of Thyroid Transcription Factor-1 and Tumor differentiation in Resected Lung Adenocarcinoma. Sci Rep 7:14222 (2017). PubMed: 29079814