细胞因子阵列-人Cytokine抗体阵列(Membrane，80 Targets) (ab133998)
概述
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产品名称
细胞因子阵列-人Cytokine抗体阵列(Membrane，80 Targets)
参阅全部 Cytokine Antibody Array 抗体芯片 -
样品类型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
检测类型
Semi-quantitative -
种属反应性
与反应: Human -
产品概述
ab133998 is for simultaneous detection of 80 Human Cytokines. Suitable for all sample types.
Targets: ENA-78, GCSF, GM-CSF, GRO, GRO-alpha, I-309, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 p40/p70, IL-13, IL-15, IFN-gamma, MCP-1, MCP-2, MCP-3, MCSF, MDC, MIG, MIP-1beta, MIP-1delta, RANTES, SCF, SDF-1, TARC, TGF-beta1, TNF-alpha, TNF-beta, EGF, IGF-I, Angiogenin, Oncostatin M, Thrombopoietin, VEGF-A, PDGF-BB, Leptin, BDNF, BLC, Ckß8-1, Eotaxin, Eotaxin-2, Eotaxin-3, FGF-4, FGF-6, FGF-7, FGF-9, Flt-3 Ligand, Fractalkine, GCP-2, GDNF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-16, IP-10, LIF, LIGHT, MCP-4, MIF, MIP-3 alpha, NAP-2, NT-3, NT-4, Osteopontin, Osteoprotegerin, PARC, PLGF, TGF-beta2, TGF-beta3, TIMP-1, TIMP-2
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (1mL/sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
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说明
If you are interested in this cytokine array, cytokine array ab133997, ab169817, ab133996, ab169811, ab169817, ab169812, ab134000, ab169803, ab134002, ab169804, ab169805, ab134003 and ab169819 may also be of interest.
A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Multiplex Protein Detectionmore details
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml 1 x 25ml 2 x 25ml 20X Wash Buffer I 1 x 10ml 1 x 20ml 1 x 10ml 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 1 x 10ml 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 1 x 10ml 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit 1 unit 1 unit Biotin-Conjugated Anti-Cytokines 2 vials 4 vials 1 vial 2 vials 4 vials Cytokine Antibody Array Membranes 1 x 4 units 2 x 4 units 1 x 2 units 1 x 4 units 2 x 4 units Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml 1 x 1.5ml 1 x 2.5ml Plastic sheets 1 unit 1 unit 1 unit 1 unit 1 unit -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133998于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Multiplex Protein Detection |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Multiplex Protein Detection
Use at an assay dependent concentration. |
图片
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Multiplex Protein Detection - Human Cytokine Antibody Array - Membrane (80 Targets) (ab133998)
Human peripheral blood cells (1x106 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.
Cells were cultured unstimulated or stimulated with 10 µg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133998.
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Quantification of Human Cytokine Antibody ArrayCells were cultured unstimulated or stimulated with 10 mg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133998. Mean pixel density was quantified using CCD camera software analysis.
数据表及文件
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SDS download
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Datasheet download
文献 (38)
ab133998 被引用在 38 文献中.
- Asurappulige HSH et al. Genotoxicity of cytokines at chemotherapy-induced 'storm' concentrations in a model of the human bone marrow. Mutagenesis N/A:N/A (2023). PubMed: 37326959
- Daniluk K et al. Effect of Melittin Complexes with Graphene and Graphene Oxide on Triple-Negative Breast Cancer Tumors Grown on Chicken Embryo Chorioallantoic Membrane. Int J Mol Sci 24:N/A (2023). PubMed: 37176095
- Kerr BN et al. Evaluation of Radiosensitization and Cytokine Modulation by Differentially PEGylated Gold Nanoparticles in Glioblastoma Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37373179
- Talvio K et al. An iPSC-derived astrocyte model of fragile X syndrome exhibits dysregulated cholesterol homeostasis. Commun Biol 6:789 (2023). PubMed: 37516746
- Lambrichts I et al. The Effect of Leukocyte- and Platelet-Rich Fibrin on Central and Peripheral Nervous System Neurons-Implications for Biomaterial Applicability. Int J Mol Sci 24:N/A (2023). PubMed: 37762617