AB133997

Cytokine Array - Human Cytokine 抗体 Array (Membrane, 42 Targets)

Name: Cytokine Array - 人Cytokine抗体阵列(Membrane, 42 Targets) (ab133997)| Abcam中文官网
Brand: Abcam Limited
SKU: AB133997
Price: 9463 CNY
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Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets)

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(47 Publications)

Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) ab133997 is a membrane based multiplex immunoassay for the simultaneous detection of 42 human cytokines in all sample types.

- More data, less sample: screen multiple cytokines with the same sample
- Global view of cytokine expression
- Chemiluminescent detection - no specific equipment required
- Highly sensitive (pg/mL)

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RNASE5, ANG, Angiogenin, Ribonuclease 5, RNase 5

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Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Multiplex Protein Detection

Lab

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Quantification of Human Cytokine Array (membrane antibody array).

Human serum from a pooled donor (n=50) sample was diluted to 50% and assayed using ab133997. Mean pixel density was quantified using CCD camera software analysis.

Multiplex Protein Detection

Lab

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Quantification of Human Cytokine Array (membrane antibody array).

Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133997. Media alone was used as a negative control. Mean pixel density was quantified using CCD camera software analysis.

Multiplex Protein Detection

Unknown

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Cytokine array testing image - Abreview.

The antibody array was used to determine the change in expression of cytokines related to cell proliferation.

One million Human breast cancer cells (MCF7) were cultured in a 10mm dish for 48 hours (in 8ml of RPMI-1640 medium) and were then treated with 5 mM Compound X (not disclosed) for 48 hours. The Abcam protocol was followed for the rest of the procedure. 1 mL of supernatant of culture medium was used for sample incubation (4°C overnight).

Rating : 4.5/5

This image is courtesy of an anonymous Abreview.

Multiplex Protein Detection

Lab

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Human serum from a pooled donor (n=50) sample was diluted to 50% and assayed using ab133997.

Multiplex Protein Detection

Lab

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Multiplex Protein Detection - Human Cytokine Array - Membrane Antibody Array.

Human peripheral blood cells (1x10⁶ cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate.

Cells were cultured unstimulated or stimulated with 10 μg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed using ab133997. Media alone was used as a negative control.

关键信息

检测类型

Semi-quantitative

反应种属

Human

靶标

ANG

target

应用

Multiplex Protein Detection

applications

样品类型

Saliva, Urine, Plasma, Cell culture extracts, Tissue Extracts, Cell culture media, Cell culture supernatant, Milk, Serum, Other biological fluids, Whole Blood, Cell Lysate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Multiplex Protein Detection": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

ab133997 is a cytokine array to be used for the simultaneous detection of 42 Human cytokines. It is suitable for all sample types.

Targets: ENA-78, GCSF, GM-CSF, GRO, GRO-alpha, I-309, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 p40/p70, IL-13, IL-15, IFN-gamma, MCP-1, MCP-2, MCP-3, MCSF, MDC, MIG, MIP-1delta, RANTES, SCF, SDF-1, TARC, TGF-beta1, TNF-alpha, TNF-beta, EGF, IGF-I, Angiogenin, Oncostatin M, Thrombopoietin, VEGF-A, PDGF BB, Leptin

Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.

If you are interested in this cytokine array, cytokine array ab133998, ab133996, ab169817, ab134003, ab134000, ab169804 and ab169805 may also be of interest.

A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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规格

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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产品实验方案

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靶点信息

Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context (PubMed : 12051708, PubMed : 1400510, PubMed : 19332886, PubMed : 20129916, PubMed : 21855800, PubMed : 23047679, PubMed : 23843625, PubMed : 2424496, PubMed : 2459697, PubMed : 2730651, PubMed : 27518564, PubMed : 28176817, PubMed : 29100074, PubMed : 29748193, PubMed : 3122207, PubMed : 32510170, PubMed : 38718836, PubMed : 8159680, PubMed : 8570639, PubMed : 8622921, PubMed : 9578571). Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus (PubMed : 29100074, PubMed : 32510170). Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs) : specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation (PubMed : 1400510, PubMed : 19332886, PubMed : 20129916, PubMed : 21855800, PubMed : 23047679, PubMed : 27518564, PubMed : 29100074, PubMed : 29748193, PubMed : 32510170, PubMed : 38718836). tiRNas also prevent formation of apoptosome, thereby promoting cell survival (By similarity). Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36 (PubMed : 3289612, PubMed : 38718836). Cleaves a subset of tRNAs, including tRNA(Ala), tRNA(Glu), tRNA(Gly), tRNA(Lys), tRNA(Val), tRNA(His), tRNA(Asp) and tRNA(Sec) (PubMed : 31582561). Under growth conditions and in differentiated cells, translocates to the nucleus and stimulates ribosomal RNA (rRNA) transcription, including that containing the initiation site sequences of 45S rRNA, thereby promoting cell growth and proliferation (PubMed : 12051708, PubMed : 15735021, PubMed : 27518564, PubMed : 29100074, PubMed : 8127865). Angiogenin induces vascularization of normal and malignant tissues via its ability to promote rRNA transcription (PubMed : 19354288, PubMed : 4074709, PubMed : 8448182). Involved in hematopoietic stem and progenitor cell (HSPC) growth and survival by promoting rRNA transcription in growth conditions and inhibiting translation in response to stress, respectively (PubMed : 27518564). Mediates the crosstalk between myeloid and intestinal epithelial cells to protect the intestinal epithelial barrier integrity : secreted by myeloid cells and promotes intestinal epithelial cells proliferation and survival (PubMed : 32510170). Also mediates osteoclast-endothelial cell crosstalk in growing bone : produced by osteoclasts and protects the neighboring vascular cells against senescence by promoting rRNA transcription (By similarity).

See full target information ANG

其他靶点

CXCL5,EGF,CSF3,CSF2,CXCL1,CXCL3,CCL1,IFNG,IGF1,IL1A,IL1B,IL10,IL12A,IL13,IL15,IL2,IL3,IL4,IL5,IL6,IL7,CXCL8,LEP,CCL2,CCL8,CCL7,CSF1,CCL22,CXCL9,CCL15,OSM,PDGFB,CCL5,KITLG,CXCL12,CCL17,TGFB1,THPO,TNF,LTA,VEGFA,CXCL2

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文献 (47)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer 24:116 PubMed40241135

2025

ADAR1-high tumor-associated macrophages induce drug resistance and are therapeutic targets in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hibiki Umeda,Kunitoshi Shigeyasu,Toshiaki Takahashi,Kazuya Moriwake,Yoshitaka Kondo,Kazuhiro Yoshida,Sho Takeda,Shuya Yano,Yuki Matsumi,Hiroyuki Kishimoto,Tomokazu Fuji,Kazuya Yasui,Hideki Yamamoto,Kosei Takagi,Masashi Kayano,Hiroyuki Michiue,Keiichiro Nakamura,Yoshiko Mori,Fuminori Teraishi,Hiroshi Tazawa,Yuzo Umeda,Shunsuke Kagawa,Ajay Goel,Toshiyoshi Fujiwara

Pituitary 28:37 PubMed40064752

2025

Cytokines and chemokines modulate the growth of pituitary adenoma/neuroendocrine tumors: preliminary results of a monocenter prospective pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Chiloiro,Pier Paolo Mattogno,Flavia Angelini,Antonella Giampietro,Alessandra Vicari,Greis Konini,Federico Valeri,Amato Infante,Natalia Cappoli,Rosalinda Calandrelli,Liverana Lauretti,Simona Gaudino,Marco Gessi,Guido Rindi,Alessandro Olivi,Laura De Marinis,Antonio Bianchi,Francesco Doglietto,Alfredo Pontecorvi

Cancer immunology, immunotherapy : CII 74:96 PubMed39904796

2025

Cancer-associated fibroblasts promote pro-tumor functions of neutrophils in pancreatic cancer via IL-8: potential suppression by pirfenidone.

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Unspecified application

Species

Unspecified reactive species

Tomohiko Yagi,Shunsuke Kagawa,Shohei Nogi,Atsuki Taniguchi,Masashi Yoshimoto,Kanto Suemori,Yasuo Nagai,Shuto Fujita,Shinji Kuroda,Satoru Kikuchi,Yoshihiko Kakiuchi,Fuminori Teraishi,Kosei Takagi,Toshiaki Ohara,Hiroshi Tazawa,Toshiyoshi Fujiwara

Theranostics 15:1966-1986 PubMed39897553

2025

TBX3 shapes an immunosuppressive microenvironment and induces immunotherapy resistance.

Applications

Unspecified application

Species

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Zhi Liu,Chunyu Zhang,Jiatong Xiao,Yunbo He,Haisu Liang,Jinliang Huang,Zhiyong Cai,Zhenglin Yi,Mingfeng Chen,Yixiao Li,Jun Zhang,Fenglian Liu,Peng Ren,Huihuang Li,Jinbo Chen,Benyi Fan,Jiao Hu,Xiongbing Zu,Dingshan Deng

Life science alliance 8: PubMed39658088

2024

Mitochondrial signatures shape phenotype switching and apoptosis in response to PLK1 inhibitors.

Applications

Unspecified application

Species

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Émilie Lavallée,Maëline Roulet-Matton,Viviane Giang,Roxana Cardona Hurtado,Dominic Chaput,Simon-Pierre Gravel

Cell death & disease 15:879 PubMed39632807

2024

Inhibition of furin in CAR macrophages directs them toward a proinflammatory phenotype and enhances their antitumor activities.

Applications

Unspecified application

Species

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Lydia Ziane-Chaouche,Antonella Raffo-Romero,Nawale Hajjaji,Firas Kobeissy,Donna Pinheiro,Soulaimane Aboulouard,Adeline Cozzani,Suman Mitra,Isabelle Fournier,Dasa Cizkova,Michel Salzet,Marie Duhamel

Advanced materials (Deerfield Beach, Fla.) 36:e2407156 PubMed39543808

2024

Biocooperative Regenerative Materials by Harnessing Blood-Clotting and Peptide Self-Assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Soraya Padilla-Lopategui,Cosimo Ligorio,Wenhuan Bu,Chengcheng Yin,Domenico Laurenza,Carlos Redondo,Robert Owen,Hongchen Sun,Felicity R A J Rose,Thomas Iskratsch,Alvaro Mata

Scientific reports 14:25815 PubMed39468126

2024

Glutamine and serum starvation alters the ATP production, oxidative stress, and abundance of mitochondrial RNAs in extracellular vesicles produced by cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Bugajova,Martina Raudenska,Klara Hanelova,Jiri Navratil,Jaromir Gumulec,Frantisek Petrlak,Tomas Vicar,Sarka Hrachovinova,Michal Masarik,David Kalfert,Marek Grega,Jan Plzak,Jan Betka,Jan Balvan

PloS one 19:e0308838 PubMed39163400

2024

Novel biomarkers in the saliva of healthy young males and females in a randomized crossover study on sedentary time: An exploratory analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Emmeline Meens Miller,Nicholas O'Rourke,Michael Jeffrey,Julia Green-Johnson,Shilpa Dogra

Cancer cell international 24:276 PubMed39103836

2024

CircUBA2 promotes the cancer stem cell-like properties of gastric cancer through upregulating STC1 via sponging miR-144-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Bin Wang,Tong-Xing Lin,Deng-Hui Fan,You-Xin Gao,Yu-Jing Chen,Yu-Kai Wu,Kai-Xiang Xu,Qing-Zhu Qiu,Ping Li,Jian-Wei Xie,Jian-Xian Lin,Qi-Yue Chen,Long-Long Cao,Chang-Ming Huang,Chao-Hui Zheng

View all publications

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Cytokine Array - Human Cytokine 抗体 Array (Membrane, 42 Targets)

Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)

Multiplex Protein Detection - Cytokine Array - Human Cytokine Antibody Array (Membrane, 42 Targets) (AB133997)