Anti-procollagen type IIA抗体[2] (ab17771)
Key features and details
- Mouse monoclonal [2] to procollagen type IIA
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-procollagen type IIA抗体[2] -
描述
小鼠单克隆抗体[2] to procollagen type IIA -
宿主
Mouse -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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表位
Epitope is located at the amino-terminal part of procollagen type IIA. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
2 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17771于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
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说明 |
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IHC-P
Use at an assay dependent concentration. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
靶标
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相关性
Collagen type II is the most abundant protein of cartilage matrix. Two isoforms exist and collagen type IIA is involved in early development of cartilage in embryos. The collagen is produced from type II procollagen when two teloterminal propeptides are digested from the precursor and released into the circulation. Procollagen type II N-terminal propeptide is considered a marker of cartilage synthesis and the normal concentration in serum is approximately 30ng/mL. -
细胞定位
Secreted -
数据库链接
- Entrez Gene: 1280 Human
- Omim: 120140 Human
- SwissProt: P02458 Human
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别名
- Alpha 1 type II collagen antibody
- Collagen II alpha 1 polypeptide antibody
- Procollagen type II alpha 1 antibody
图片
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ab17771, at a 1/1000 dilution, staining fetal bone anlage in the hand (around week 10) by IHC-P.
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ab17771, at a 1/1000 dilution, staining specific tumour cells of biopsy from a chondroblastoma by IHC-P. The antibody was detected with an HRP conjugated Goat anti-Mouse antibody.
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ab17771 staining mouse eye tissue sections -- conjunctiva and limbus by IHC-P. The tissue was fixed with paraformaldehyde and antigen retrieval was heat mediated using citrate buffer. The blocking agent was 1% TSA at 25°C for 1 hour. The primary antibody was incubated (undiluted) at 25°C for 15 hours. A biotinylated anti-mouse was used as the secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab17771 被引用在 4 文献中.
- Chen L et al. Overexpression of long non-coding RNA AP001505.9 inhibits human hyaline chondrocyte dedifferentiation. Aging (Albany NY) 13:11433-11454 (2021). PubMed: 33839696
- Qiu F et al. Development and Verification of an Immune-Based Gene Signature for Risk Stratification and Immunotherapeutic Efficacy Assessment in Gastric Cancer. Dis Markers 2021:4251763 (2021). PubMed: 34804261
- Olderøy MØ et al. Biochemical and structural characterization of neocartilage formed by mesenchymal stem cells in alginate hydrogels. PLoS One 9:e91662 (2014). IHC-P ; Human . PubMed: 24626259
- Lee CH et al. Protein-releasing polymeric scaffolds induce fibrochondrocytic differentiation of endogenous cells for knee meniscus regeneration in sheep. Sci Transl Med 6:266ra171 (2014). PubMed: 25504882