Pro-Apoptosis Bcl Family抗体Sampler组合(ab228527)
概述
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产品名称
Pro-Apoptosis Bcl Family抗体Sampler组合 -
产品概述
ab228527, Apoptosis Antibody Sampler Panel, is a collection of 1 anti-rabbit (HRP) secondary antibody and 5 recombinant rabbit monoclonal antibodies against Bad, Bax, Bcl-2, Bcl-XL, and MCL1 provided in 10 µl trial sizes.
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说明
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Directly conjugated versions of our antibodies are available and ready to use for multicolor flow cytometry or immunocytochemistry analysis. Carrier-free formulations are also available for easy conjugation to labels of your choice. Please refer to the ‘Associated products’ section below.
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 kit ab32445 - Anti-Bad antibody [Y208] 1 x 10µl ab32503 - Anti-Bax antibody [E63] 1 x 10µl ab182858 - Anti-Bcl-2 antibody [EPR17509] 1 x 10µl ab32370 - Anti-Bcl-XL antibody [E18] 1 x 10µl ab32087 - Anti-MCL1 antibody [Y37] 1 x 10µl ab205718 - Goat Anti-Rabbit IgG H+L (HRP) 1 x 100µg
相关产品
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Compatible Secondaries
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Related Products
- Anti-MCL1 antibody [Y37] - BSA and Azide free (ab186822)
- Alexa Fluor® 647 Anti-MCL1 antibody [Y37] (ab197035)
- Alexa Fluor® 488 Anti-MCL1 antibody [Y37] (ab197529)
- Anti-Bcl-XL antibody [E18] - BSA and Azide free (ab199099)
- Biotin Anti-Bad antibody [Y208] (ab199754)
- PE Anti-Bcl-XL antibody [E18] (ab208747)
- PE Anti-MCL1 antibody [Y37] (ab209289)
- Anti-Bax antibody [E63] - BSA and Azide free (ab216985)
- Anti-Bcl-2 antibody [EPR17509] - BSA and Azide free (ab219608)
- Anti-Bad antibody [Y208] - BSA and Azide free (ab220116)
- PE Anti-CD28 antibody [E18] (ab64638)
图片
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Immunocytochemistry/Immunofluorescence analysis of H1299 cells labelling MCL1 with unpurified ab32087. Cells were PFA-fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 3% Serum for 1 hour at 24°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 24°C. The secondary antibody was an Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/2000. DAPI (blue) was used as the nuclear counterstain.
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Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Bcl-2 with ab182858 at 1/1000 followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.
Cytoplasm, nuclear membrane and nucleus staining on lymphocytes and cancer cells of Human endometrial cancer tissue is observed.
Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody followed by ab97051 at 1/500. -
Immunohistochemistry of human primary melanoma, staining Bcl-XL (red) with unpurified ab32370.
Antigen retrieval was performed in EDTA/Tris buffer (pH 8) before being blocked with 10%NGS for one hour at room temperature. Samples were incubated with primary antibody (1/50) at room temperature for one hour. An AlexaFluor®-conjugated anti-rabbit IgG was used as the secondary antibody. -
IHC image of ab32503 staining Bax in rat kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32503, 1:250 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemical analysis of 2% paraformaldehyde-fixed,0.1% Triton-X100 permeabilized RAW 264.7 labeling Bad with ab32445 at 1/250dilution,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) secondary antibody at 1/1000 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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