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PEG is the common abbreviation for polyethylene glycol – or, more properly, poly (ethylene glycol) – which refers to a chemical compound composed of repeating ethylene glycol units. PEG is which is water-soluble, non-toxic, non-antigenic, and biocompatible. Purified PEG is most defined by their molecular weight (MW) ranges.
For example, PEG600 is PEG polymer with MW of 600.
PEG 10K or PEG 10,000 denotes a mixture of PEG molecules (about 195-265 PEG molecules) having an average MW of 10,000 g/mol.
PEG has several chemical properties that make it especially useful in various biological, chemical and pharmaceutical settings:
Due to these unique properties, PEG is commonly used to PEGlyate various protein and drug molecules (1).
An Anti-PEG antibody can be used to monitor a drug’s pharmacokinetics, including distribution, metabolism and excretion. Also, it can be used for the quality control of pegylated molecules in ELISA, WB and IHC.
We offer various PEG antibodies and kits for PEG detection, including our high quality rabbit monoclonal PEG antibodies.
For PEG antibody to PEG methoxy group, please see Anti-Polyethylene glycol antibody [PEG-B-47] (ab51257).
For PEG antibody to PEG methoxy group, conjugated to Biotin, please see Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) (ab53449).
For PEG antibody to PEG backbone, please see Anti-Polyethylene glycol antibody [PEG-2-128] ( ab133471)
For PEG ELISA kit, please see PEG ELISA Kit (ab138914).
Yes, ab51257 is used in the kit as the capture antibody in the kit and it has been shown to detect both linear and branched PEG.
Yes, ab53449 is purified before biotinylation. Protein A was used to purify this antibody.
If there is a PEG molecule present, Abcam PEG antibodies will detect the PEGylated molecule. Abcam PEG products are independent of sample or species used.
It works in all species as long as a PEG molecule is present.
We recommend our standard IHC protocol, with a dilution factor or 1:50 - 1:100.
What is the smallest PEG molecule your PEG antibodies can detect? What is the largest PEG molecule your PEG antibodies can detect?
Our RabMAb PEG ELISA kit (ab138914) cannot be used in detecting free PEG.
Since free PEG and conjugated PEG have different MW, these two forms can be distinguished by western blot using anti-PEG (backbone) antibody, ab133471.
Since ab133471 recognizes PEG backbone, PEG is not required to be bound to other molecules. We have direct ELISA data using PEG5K, 6K, 20K to confirm this.
Our RabMAb PEG antibodies are provided as 100ug, so you can calculate the volume using the following calculation:
(0.1mg) divided by the current concentration of antibody (mg/ml)
= volume (ml)
Use antigen/antibody diluent buffer in the kit. Any PBS based buffer with BSA and/or Tween can be used.
The PEG ELISA kit (ab138914) operates on the basis of competition between the enzyme (HRP) conjugated PEG and PEG labeled molecules for a limited number of binding sites on the surface of 96-wells coated with Anti-PEG antibody.
The extent of color development resulted from interaction between HRP and substrate TMB is inversely proportional to the amount of PEGylated molecules in the sample.
We have not tested fluorocarbons containing PEG so we do not know for certain. However, we tested H2N-PEG6000-OCH3 using the kit and it did not compete with HRP-PEG.
Ab133471 cannot be used with our PEG ELISA kit.
The numbers that are often included in the names of PEGs indicate their average molecular weights, e.g., a PEG with n=9 would have an average molecular weight of approximately 400 daltons and would be labeled PEG 400. Most PEGs include molecules with a distribution of molecular weights; i.e., they are polydisperse.
Therefore, N/Daltons is 9/400. You can use this ratio to figure out either N or size in Dalton as long as you have either N or Dalton value.
About 5 moles of PEG5K are conjugated to 1 mole of BSA.