概述

  • 产品名称
    Prestained蛋白Ladder – Broad molecular weight (10-245 kDa)
    参阅全部 Prestained Protein Ladder 试剂
  • 描述
    Prestained蛋白Ladder - Broad molecular weight (10-245 kDa)
  • 经测试应用
    适用于: SDS-PAGE, WBmore details
  • 常规说明

    This product was previously called Prism Ultra Protein Ladder (10-245 kDa).

    ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).

    ab116028 is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

    Key product features:

    • Broad range: 10-245 kDa
    • Ready-to-use: Supplied in a loading buffer for direct loading on gels.
    • Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.
    • Sharp bands
    • Recommended loading: ~1.5 - 2.5µl

     

    Review other ladders in the guide to unstained and prestained protein ladder products.

     

     


    Storage: Stable for up to 3 months at 4°C. For long term storage, store at -20°C.

性能

  • 形式
    Liquid
  • 存放说明
    Please see notes section.
  • 存储溶液
    Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% SDS, 0.02% DTT, 15% Glycerol
  • Concentration information loading...
  • 研究领域
  • 别名
    • Prism protein ladder
    • SDS PAGE protein ladder prestained
    • WB prestained protein ladder
    • Western blot prestained protein ladder

应用

Our Abpromise guarantee covers the use of ab116028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
SDS-PAGE Use at an assay dependent concentration. 3 µl or 5 µl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.
WB Use at an assay dependent concentration. ~1.5-2.5 µl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.

图片

  • Gel 1: Tris-Glycine (~4-20%), Gel 2: Bis-Tris (10%) MOPS buffer, Gel 3: Bis-Tris (10%) MES buffer.

    Pre-stained molecular weight standards have a differing mobility and as a consequence varying apparent molecular weight when run in distinct SDS-PAGE buffer systems. The variance in pH of alternative buffers affects the charge of the labelled protein standard and its binding capacity for SDS. The apparent molecular weight of this marker has been determined by calibration against an unstained ladder in each electrophoresis condition.

文献

This product has been referenced in:
  • Singh AK  et al. Curcumin Quantum Dots Mediated Degradation of Bacterial Biofilms. Front Microbiol 8:1517 (2017). Read more (PubMed: 28848526) »
  • Wang L  et al. Upregulation of microRNA-181b inhibits CCL18-induced breast cancer cell metastasis and invasion via the NF-?B signaling pathway. Oncol Lett 12:4411-4418 (2016). Read more (PubMed: 28105154) »
See all 4 Publications for this product

客户评价及客户问答

1-4 of 4 Abreviews or Q&A

Prestained ladder works at LICOR

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The ladder works perfectly at 2.5 ul (down to 1 ul) and also shows nice crisps bands not only on the nitrocellulose membrane but also when analysed at the LICOR. Same if not even a touch better results then comparable ladders from more expensive competitors.

Sebastian Bohm

Verified customer

提交于 Jun 22 2018

One of the best Pre stained protein ladder

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I have used 1.8 ul per well and get a clear band on transfer to membrane.

Vicky

Verified customer

提交于 Oct 10 2016

Answer

The apparent sizes will be the same as the ones in the second gel on the online datasheet, which is a 10% Bis-Tris gel, MOPS running buffer. The distribution will be different, with a little more separation of the higher molecular weight proteins at the 4% end of your gel compared to the 10% gel on the datasheet.

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Question
Answer

I can confirm that ab116027, ab116028, and ab116029 do not contain histone tagged proteins.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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