FITC荧光Anti-PR3抗体[PR3G-2] (ab65255)
Key features and details
- FITC Mouse monoclonal [PR3G-2] to PR3
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
FITC荧光Anti-PR3抗体[PR3G-2]
参阅全部 PR3 一抗 -
描述
FITC荧光小鼠单克隆抗体[PR3G-2] to PR3 -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
This antibody does not inhibit the biological activity of PR3. -
经测试应用
适用于: Flow Cyt (Intra)more details -
种属反应性
与反应: Human -
免疫原
Tissue/ cell preparation (Human): a crude granule extract.
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阳性对照
- Flow Cyt (Intra): THP1 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
PR3G-2 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65255于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use 0.5µg for 106 cells.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt (Intra)
Use 0.5µg for 106 cells. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. -
序列相似性
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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数据库链接
- Entrez Gene: 5657 Human
- Omim: 177020 Human
- SwissProt: P24158 Human
- Unigene: 928 Human
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别名
- ACPA antibody
- AGP 7 antibody
- AGP7 antibody
see all
图片
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Overlay histogram showing THP1 cells stained with ab65255 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab65255, 0.5µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [MOPC-21] (FITC) (ab18435, 0.5µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab65255 被引用在 1 文献中.
- Liu H et al. Proteinase 3 expression on the neutrophils of patients with paroxysmal nocturnal hemoglobinuria. Exp Ther Med 15:2525-2532 (2018). PubMed: 29467851