重组Anti-PPP2R5E抗体[EPR17146] (ab198290)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17146] to PPP2R5E
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PPP2R5E抗体[EPR17146]
参阅全部 PPP2R5E 一抗 -
描述
兔单克隆抗体[EPR17146] to PPP2R5E -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, HEK-293T, Daudi, C6 and NIH/3T3 cell lysates; Human pancreas and fetal brain tissue lysate; Mouse and rat brain tissue lysates. ICC/IF: C6 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17146 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab198290于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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ICC/IF |
1/500.
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说明 |
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WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
ICC/IF
1/500. |
靶标
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功能
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. -
序列相似性
Belongs to the phosphatase 2A regulatory subunit B56 family. -
翻译后修饰
Phosphorylated on serine residues. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5529 Human
- Entrez Gene: 26932 Mouse
- Entrez Gene: 299147 Rat
- Omim: 601647 Human
- SwissProt: Q16537 Human
- SwissProt: Q61151 Mouse
- Unigene: 334868 Human
- Unigene: 259626 Mouse
see all -
别名
- 2A5E_HUMAN antibody
- Epsilon isoform of regulatory subunit B56 protein phosphatase 2A antibody
- PP2A B subunit B' epsilon antibody
see all
图片
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All lanes : Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PPP2R5E knockout HeLa cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 55 kDaLanes 1-4: Merged signal (red and green). Green - ab198290 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.
ab198290 Anti-PPP2R5E antibody [EPR17146] was shown to specifically react with PPP2R5E in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265637 (knockout cell lysate ab258135) was used. Wild-type and PPP2R5E knockout samples were subjected to SDS-PAGE. ab198290 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution + 293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDaBlocking/dilution buffer: 5% NFDM/TBST.
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Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution + Human fetal brain lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDaBlocking/dilution buffer: 5% NFDM/TBST.
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Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution + Human pancreas lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDaBlocking/dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-PPP2R5E antibody [EPR17146] (ab198290) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : C6 (Rat glial tumor cell) lysate
Lane 4 : NIH/3T3 (Mouse embryo fibroblast cells) lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDaBlocking/dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor) cells labeling PPP2R5E with ab198290 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on C6 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1 - ab198290 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab198290 尚未被引用在任何文献中。