Anti-PPP2R5B抗体(ab1366)
概述
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产品名称
Anti-PPP2R5B抗体
参阅全部 PPP2R5B 一抗 -
描述
山羊多克隆抗体to PPP2R5B -
宿主
Goat -
经测试应用
适用于: ELISA, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Dog
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免疫原
Synthetic peptide: QRLTPQVAASGGQS, corresponding to C terminal amino acids 484-497 of Human PPP2R5B.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab1366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | ||
| WB |
应用说明
ELISA: Detection limit dilution 1/2000.
WB: Use at a concentration of 2 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 2 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 on the 'Thr-308' and 'Ser-373' residues. -
组织特异性
Highest expression in brain. -
序列相似性
Belongs to the phosphatase 2A regulatory subunit B56 family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 5526 Human
- Entrez Gene: 225849 Mouse
- Entrez Gene: 309179 Rat
- NCBI: 5453952 Human
- Omim: 601644 Human
- SwissProt: Q15173 Human
- Unigene: 75199 Human
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别名
- 2A5B_HUMAN antibody
- B56B antibody
- Beta isoform of regulatory subunit B56 protein phosphatase 2A antibody
see all
图片
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Western blot - Anti-PPP2R5B antibody (ab1366)Anti-PPP2R5B antibody (ab1366) at 2 µg/ml + Human cerebellum lysate at 35 µg
Predicted band size: 55 kDa
Observed band size: 55 kDa
数据表及文件
文献
This product has been referenced in:
- DeGrande ST et al. Molecular mechanisms underlying cardiac protein phosphatase 2A regulation in heart. J Biol Chem 288:1032-46 (2013). WB ; Human, Mouse, Rat, Dog . Read more (PubMed: 23204520) »
