概述

  • 产品名称
    Anti-PPP2R5B抗体
    参阅全部 PPP2R5B 一抗
  • 描述
    山羊多克隆抗体to PPP2R5B
  • 宿主
    Goat
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide: QRLTPQVAASGGQS, corresponding to C terminal amino acids 484-497 of Human PPP2R5B.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

应用

Our Abpromise guarantee covers the use of ab1366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    ELISA: Detection limit dilution 1/2000.
    WB: Use at a concentration of 2 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    • 功能
      The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 on the 'Thr-308' and 'Ser-373' residues.
    • 组织特异性
      Highest expression in brain.
    • 序列相似性
      Belongs to the phosphatase 2A regulatory subunit B56 family.
    • 细胞定位
      Cytoplasm.
    • Information by UniProt
    • 数据库链接
      • 别名
        • 2A5B_HUMAN antibody
        • B56B antibody
        • Beta isoform of regulatory subunit B56 protein phosphatase 2A antibody
        • FLJ35411 antibody
        • PP2A B subunit B' beta isoform antibody
        • PP2A B subunit B56 beta isoform antibody
        • PP2A B subunit isoform B' beta antibody
        • PP2A B subunit isoform B''-beta antibody
        • PP2A B subunit isoform B56-beta antibody
        • PP2A B subunit isoform PR61-beta antibody
        • PP2A B subunit isoform R5-beta antibody
        • PP2A B subunit PR61 beta isoform antibody
        • PP2A B subunit R5 beta isoform antibody
        • PPP2R5B antibody
        • PR 61B antibody
        • PR61B antibody
        • Protein phosphatase 2 regulatory subunit B (B56) beta antibody
        • Protein phosphatase 2 regulatory subunit B (B56) beta isoform antibody
        • Protein phosphatase 2 regulatory subunit B' beta antibody
        • Protein phosphatase 2 regulatory subunit B' beta isoform antibody
        • Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit beta isoform antibody
        • Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform antibody
        see all

      图片

      • Western blot - Anti-PPP2R5B antibody (ab1366)
        Western blot - Anti-PPP2R5B antibody (ab1366)
        Anti-PPP2R5B antibody (ab1366) at 2 µg/ml + Human cerebellum lysate at 35 µg

        Predicted band size: 55 kDa
        Observed band size: 55 kDa

      实验方案

      文献

      This product has been referenced in:
      • DeGrande ST  et al. Molecular mechanisms underlying cardiac protein phosphatase 2A regulation in heart. J Biol Chem 288:1032-46 (2013). WB ; Human, Mouse, Rat, Dog . Read more (PubMed: 23204520) »

      See 1 Publication for this product

      发表研究结果有使用 ab1366？请让我们知道，以便我们可以引用本数据表中的参考文章。

      客户评价及客户问答

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      1 Review or Q&A

      10
      Votes: Highest First

      Immunocytochemistry/ Immunofluorescence abreview for Anti-PPP2R5B antibody

       Excellent
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HEK293T)
      Permeabilization
      Yes - 0.1% Triton X-100
      Specification
      HEK293T
      Blocking step
      Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More
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      提交于 Mar 31 2017

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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