Key features and details
- Goat polyclonal to PPP2R5A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab1084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
功能The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
组织特异性Widely expressed with the highest expression in heart and skeletal muscle.
序列相似性Belongs to the phosphatase 2A regulatory subunit B56 family.
翻译后修饰Phosphorylated on serine residues.
细胞定位Cytoplasm. Nucleus. Chromosome > centromere. From mitotic prophase to metaphase, localizes at the inner centromere between a pair of sister kinetochores. Decreased expression at the onset of anaphase.
- Information by UniProt
- 2A5A_HUMAN antibody
- B56A antibody
- MGC131915 antibody
ab1084 被引用在 1 文献中.
- Mannava S et al. PP2A-B56a controls oncogene-induced senescence in normal and tumor human melanocytic cells. Oncogene 31:1484-92 (2012). PubMed: 21822300