Anti-PPAR gamma抗体(ab59256)
Key features and details
- Rabbit polyclonal to PPAR gamma
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PPAR gamma抗体
参阅全部 PPAR gamma 一抗 -
描述
兔多克隆抗体to PPAR gamma -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic non-phosphopeptide derived from human PPAR gamma around the phosphorylation site of serine 112 (P-A-SP-P-P).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab59256 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59256于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 57 kDa.
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ICC/IF |
Use a concentration of 5 µg/ml.
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IHC-P | (2) |
1/50 - 1/100.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 57 kDa. |
ICC/IF
Use a concentration of 5 µg/ml. |
IHC-P
1/50 - 1/100. |
靶标
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功能
Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. -
组织特异性
Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary. -
疾病相关
Note=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.
Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.
Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.
Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5468 Human
- Omim: 601487 Human
- SwissProt: P37231 Human
- Unigene: 162646 Human
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别名
- CIMT1 antibody
- GLM1 antibody
- NR1C3 antibody
see all
图片
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All lanes : Anti-PPAR gamma antibody (ab59256) at 1/500 dilution
Lane 1 : HUVEC (human umbilical vein endothelial cell line) whole cell lysate
Lane 2 : HUVEC (blocked with immunizing peptide), whole cell lysate
Predicted band size: 57 kDa -
ab59256, at 1/50 dilution, staining PPAR gamma in paraffin embedded human placenta tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
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ICC/IF image of ab59256 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59256, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (60)
ab59256 被引用在 60 文献中.
- Farini A et al. Microbiota dysbiosis influences immune system and muscle pathophysiology of dystrophin-deficient mice. EMBO Mol Med 15:e16244 (2023). PubMed: 36533294
- Yu X et al. LncRNA SNHG1 modulates adipogenic differentiation of BMSCs by promoting DNMT1 mediated Opg hypermethylation via interacting with PTBP1. J Cell Mol Med 26:60-74 (2022). PubMed: 34854215
- Shao W et al. Cytoplasmic Colocalization of RXRα and PPARγ as an Independent Negative Prognosticator for Breast Cancer Patients. Cells 11:N/A (2022). PubMed: 35406808
- Luo Y et al. Chronic Intermittent Hypoxia Exposure Alternative to Exercise Alleviates High-Fat-Diet-Induced Obesity and Fatty Liver. Int J Mol Sci 23:N/A (2022). PubMed: 35563600
- Garcia-Urkia N et al. Enhanced Adipogenic Differentiation of Human Dental Pulp Stem Cells in Enzymatically Decellularized Adipose Tissue Solid Foams. Biology (Basel) 11:N/A (2022). PubMed: 35892955