Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
1 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Odyssey Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Li-Cor 926-68073 IRDye® 680RD Donkey anti-Rabbit I
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 680
Detection
Detection method
Li-Cor
Exposure
2 second(s)
Bands
Specific: 53 kDa Non-specific: 70 kDa
Positive control
my sample
Negative control
my sample
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Xiaofeng Xin
Verified customer
提交于 May 13 2013