Key features and details
- Rabbit polyclonal to PPAR delta
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Sheep, Human, Pig
- Isotype: IgG
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Constituents: BSA, Tris buffered saline, ddH20
Contains 5 mg/ml BSA.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab23673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 4 µg/ml.|
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 50 kDa. Nuclear extraction may be required in some samples. Detects a band of approximately 50 kDa in human samples, and an additional smaller size of PPAR delta is detected in some murine samples at approximately 40 kDa.|
功能Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
组织特异性Ubiquitous with maximal levels in placenta and skeletal muscle.
序列相似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
- Information by UniProt
- FAAR antibody
- MGC3931 antibody
- NR1C2 antibody
Anti-PPAR delta antibody (ab23673) at 1/1000 dilution + Rat brain tissue lysate at 100 µg
HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Immunofluorescent staining of HT-29 cells (ab3952) (nuclear staining) using ab23673.
All lanes : Anti-PPAR delta antibody (ab23673)
Lane 1 : Human cerebral cortex (30ug)
Lane 2 : Murine liver (30ug)
Predicted band size: 50 kDa
ab23673 recognises PPAR delta at 50kDa from human samples. An additional smaller size of PPAR delta (~40kDa) is also detected in certain murine tissues.
ab23673 被引用在 27 文献中.
- Lee K et al. Gomisin N from Schisandra chinensis Ameliorates Lipid Accumulation and Induces a Brown Fat-Like Phenotype through AMP-Activated Protein Kinase in 3T3-L1 Adipocytes. Int J Mol Sci 21:N/A (2020). PubMed: 32245100
- Zhong R et al. Potential Role of circPVT1 as a proliferative factor and treatment target in esophageal carcinoma. Cancer Cell Int 19:267 (2019). PubMed: 31636510
- Yang Z et al. A pilot study on polycystic ovarian syndrome caused by neonatal exposure to tributyltin and bisphenol A in rats. Chemosphere 231:151-160 (2019). PubMed: 31129395
- Shen D et al. A novel peptide suppresses adipogenic differentiation through activation of the AMPK pathway. Biochem Biophys Res Commun 510:395-402 (2019). PubMed: 30717977
- Zhao L et al. PPAR signaling pathway in the first trimester placenta from in vitro fertilization and embryo transfer. Biomed Pharmacother 118:109251 (2019). PubMed: 31351426