存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Constituents: BSA, Tris buffered saline, ddH20
Contains 5 mg/ml BSA.
Concentration information loading...
纯度Immunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab23673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 4 µg/ml.|
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 50 kDa.Can be blocked with Human PPAR delta peptide (ab104373). Nuclear extraction may be required in some samples. Detects a band of approximately 50 kDa in human samples, and an additional smaller size of PPAR delta is detected in some murine samples at approximately 40 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
功能Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
组织特异性Ubiquitous with maximal levels in placenta and skeletal muscle.
序列相似性Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
- Information by UniProt
- FAAR antibody
- MGC3931 antibody
- NR1C2 antibody
Anti-PPAR delta antibody (ab23673) at 1/1000 dilution + Rat brain tissue lysate at 100 µg
HRP-conjugate Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Immunofluorescent staining of HT-29 cells (ab3952) (nuclear staining) using ab23673.
Immunohistochemical analysis of paraffin-embedded pig kidney tissue labeling PPAR delta with ab23673 at 1/100 dilution,followed by Goat Anti-Rabbit IgG
ab23673 staining PPAR delta in Rat brain tissue (ab29475) sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with acetone, permeablized with methanol and blocked with 5% BSA for 1 hour at 37°C. The sample was incubated with primary antibody (1/100 in PBS) at 4°C for 18 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit (ab150077) polyclonal (1/200) was used as the secondary antibody.
ab23673 staining mouse brain tissue sections by IHC-Fr. Sections were PFA fixed and permeabilized in Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 48 hours at 4°C. A FITC conjugated goat anti-rabbit antibody was used as the secondary.
All lanes : Anti-PPAR delta antibody (ab23673)
Lane 1 : Human cerebral cortex (30ug)
Lane 2 : Murine liver (30ug)
Predicted band size: 50 kDa
ab23673 recognises PPAR delta at 50kDa from human samples. An additional smaller size of PPAR delta (~40kDa) is also detected in certain murine tissues.
This product has been referenced in:
- Shen D et al. A novel peptide suppresses adipogenic differentiation through activation of the AMPK pathway. Biochem Biophys Res Commun 510:395-402 (2019). Read more (PubMed: 30717977) »
- Kaup D et al. The carnitine status does not affect the contractile and metabolic phenotype of skeletal muscle in pigs. Nutr Metab (Lond) 15:2 (2018). WB ; Pig . Read more (PubMed: 29344054) »