概述

  • 产品名称
    Anti-PPAR delta抗体
    参阅全部 PPAR delta 一抗
  • 描述
    兔多克隆抗体to PPAR delta
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Pig
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 129-378 of Human PPAR delta.

  • 阳性对照
    • Molt-4 and Mouse brown adipose tissue whole cell lysates; A549 Xenograft tissue.

性能

应用

Our Abpromise guarantee covers the use of ab137724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)

靶标

  • 功能
    Ligand-activated transcription factor. Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Has a preference for poly-unsaturated fatty acids, such as gamma-linoleic acid and eicosapentanoic acid. Once activated by a ligand, the receptor binds to promoter elements of target genes. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the acyl-CoA oxidase gene. Decreases expression of NPC1L1 once activated by a ligand.
  • 组织特异性
    Ubiquitous with maximal levels in placenta and skeletal muscle.
  • 序列相似性
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FAAR antibody
    • MGC3931 antibody
    • NR1C2 antibody
    • NUC1 antibody
    • NUCI antibody
    • NUCII antibody
    • Nuclear hormone receptor 1 antibody
    • Nuclear receptor subfamily 1 group C member 2 antibody
    • Peroxisome proliferative activated receptor delta antibody
    • Peroxisome proliferator-activated receptor beta (PPAR-beta) antibody
    • Peroxisome proliferator-activated receptor beta antibody
    • Peroxisome proliferator-activated receptor delta antibody
    • PPAR beta antibody
    • PPAR-beta antibody
    • PPAR-delta antibody
    • PPAR-ß antibody
    • PPARB antibody
    • ppard antibody
    • PPARD_HUMAN antibody
    see all

图片

  • Anti-PPAR delta antibody (ab137724) at 1/1000 dilution + Molt4 whole cell lysate at 30 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Anti-PPAR delta antibody (ab137724) at 1/1000 dilution + Mouse brown adipose tissue whole cell lysate
    at 50 µg

    Predicted band size: 50 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labelling PPAR delta with ab137724 at 1/100 dilution.

文献

This product has been referenced in:
  • Li XH  et al. Neuroprotective effects of sodium hydrosulfide against ß-amyloid-induced neurotoxicity. Int J Mol Med 38:1152-60 (2016). WB ; Rat . Read more (PubMed: 27511125) »
  • Maurya SK  et al. Sarcolipin Is a Key Determinant of the Basal Metabolic Rate, and Its Overexpression Enhances Energy Expenditure and Resistance against Diet-induced Obesity. J Biol Chem 290:10840-9 (2015). Read more (PubMed: 25713078) »

See all 2 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Pig Cell lysate - whole cell (RPE)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
RPE
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

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提交于 Jun 29 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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