Application
Western blot
Sample
Mouse Tissue lysate - nuclear (Liver nuclei lysates from Ppara null and wildtype)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Liver nuclei lysates from Ppara null and wildtype
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk/TBST
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: HRP anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
Ppara knockout mice
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 52 kDa Non-specific: lots kDa
Positive control
wildtype mice
Additional data
Additional Notes
Good specificity towards Ppara. Lots of high molecular weight non-specific bands.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Chad Brocker
Verified customer
提交于 Apr 17 2015