Anti-PP2A alpha + beta抗体(ab137849)
Key features and details
- Rabbit polyclonal to PP2A alpha + beta
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PP2A alpha + beta抗体
参阅全部 PP2A alpha + beta 一抗 -
描述
兔多克隆抗体to PP2A alpha + beta -
宿主
Rabbit -
特异性
ab137849 does cross react with PP2A beta -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis -
免疫原
Synthetic peptide corresponding to Human PP2A-alpha aa 247-259.
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阳性对照
- 293T, A431, H1299, HeLaS3, Molt4, Raji and mouse brain lysates; HeLa cell; A549 xenograft
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137849于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
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ICC/IF | (1) |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer ( pH8.0) may be used. |
靶标
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功能
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'. -
序列相似性
Belongs to the PPP phosphatase family. PP-1 subfamily. -
翻译后修饰
Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle.
Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. -
细胞定位
Cytoplasm. Nucleus. Chromosome > centromere. Cytoplasm > cytoskeleton > spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2. - Information by UniProt
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数据库链接
- Entrez Gene: 396021 Chicken
- Entrez Gene: 416318 Chicken
- Entrez Gene: 5515 Human
- Entrez Gene: 5516 Human
- Entrez Gene: 19052 Mouse
- Entrez Gene: 19053 Mouse
- Entrez Gene: 397136 Pig
- Entrez Gene: 397656 Pig
see all -
别名
- PP2A A antibody
- PP2A alpha antibody
- PP2A B antibody
see all
图片
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All lanes : Anti-PP2A alpha + beta antibody (ab137849) at 1/1500 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Molt4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
10% SDS PAGE -
Anti-PP2A alpha + beta antibody (ab137849) at 1/10000 dilution + mouse brain whole cell lysate at 50 µg
Predicted band size: 35 kDa
10% SDS PAGE -
Confocal immunofluorescence analysis of paraformaldehyde fixed HeLa cell labeling PP2A alpha with ab137849 (green) at a 1/500 dilution. Alpha tubulin filaments were labeled with an Alpha tubulin antibody (red) at a 1/2000 dilution.
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Immunohistochemical analysis of paraffin embedded A549 xenograft, labeling PP2A alpha using ab137849 at a 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab137849 被引用在 2 文献中.
- Yang Y et al. Skullcapflavone I suppresses proliferation of human lung cancer cells via down-regulating microRNA-21. Exp Mol Pathol 110:104285 (2019). PubMed: 31301304
- Kang HR et al. Mg-Al and Zn-Al Layered Double Hydroxides Promote Dynamic Expression of Marker Genes in Osteogenic Differentiation by Modulating Mitogen-Activated Protein Kinases. Adv Healthc Mater 7:N/A (2018). PubMed: 29280352