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|Cell culture supernatant||104.8||97% - 113%|
|Serum||99.39||86% - 112%|
|Plasma||80.15||67% - 104%|
The Porcine VEGF-A ELISA kit (ab218298) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Porcine VEGF-A in serum (Porcine VEGF-A concentration is low in normal serum and plasma samples and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for VEGF-A coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF-A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Porcine VEGF-A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF-A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is Measured at 450 nm.
|组件||1 x 96 tests|
|20X Wash Buffer||1 x 25ml|
|300X Streptavidin-Conjugated HRP||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Porcine VEGF-A coated Microplate (12 x 8 wells)||1 x 96 tests|
|Assay Diluent C||1 x 30ml|
|Biotinylated Anti-Pig VEGF-A Detection Antibody||2 vials|
|Lyophilized Pig VEGF-A Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab218298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab218298 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"