概述

  • 产品名称
    Porcine VEGF-A ELISA试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    1 < 10%
    批次间
    样品 n Mean SD CV%
    1 < 12%
  • 样品类型
    Cell culture supernatant, Serum, Plasma
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    40 pg/ml
  • 范围
    40 pg/ml - 10000 pg/ml
  • 回收率

    特定样本回收率
    样品类型 平均% 范围
    Cell culture supernatant 104.8 97% - 113%
    Serum 99.39 86% - 112%
    Plasma 80.15 67% - 104%

  • 检测时间
    4h 45m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Pig
  • 产品概述

    The Porcine VEGF-A ELISA kit (ab218298) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Porcine VEGF-A in serum (Porcine VEGF-A concentration is low in normal serum and plasma samples and may not be detectable in this assay), plasma and cell culture supernatants. 


    This assay employs an antibody specific for VEGF-A coated on a 96-well plate. Standards and samples are pipetted into the wells and VEGF-A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Porcine VEGF-A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of VEGF-A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is Measured at 450 nm.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Pre-coated microplate (12 x 8 well strips)

性能

应用

Our Abpromise guarantee covers the use of ab218298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Porcine VEGF-A ELISA kit (ab218298) Standard curve.

实验方案

文献

ab218298 has not yet been referenced specifically in any publications.

客户评价及客户问答

There are currently no Customer reviews or Questions for ab218298.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册