Name: Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin)
Brand: RabMAb
SKU: ab53449
Rating: 5 (1 reviews)

产品名称

Anti-Polyethylene glycol抗体[PEG-B-47b] (Biotin)
参阅全部 Polyethylene glycol 一抗
描述

兔单克隆抗体[PEG-B-47b] to Polyethylene glycol (Biotin)
宿主

Rabbit
偶联物

Biotin
经测试应用

适用于: IHC-P, Sandwich ELISA, WB, ELISAmore details
免疫原

Chemical/ Small Molecule corresponding to Polyethylene glycol conjugated to Keyhole Limpet Haemocyanin (KLH). KLH-PEG with a terminal methoxy group.

Run BLAST with Run BLAST with
阳性对照
- WB: BSA-PEG5K.
常规说明

The smallest PEG molecule which our antibody can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab53449 detects both linear and branched PEG based on our in-house ELISA testing.

Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents. Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents
when performing ELISA using anti-PEG products.

Learn more about PEG RabMAb^® primary antibodies and kit - PEG RabMAb Product Portal Page
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

PEG-B-47b
同种型

IgG
研究领域

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG (Biotin) - Isotype Control (ab200208)
Positive Controls
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

Our Abpromise guarantee covers the use of ab53449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Ab评论	说明
IHC-P		1/250. For unpurified use at 1/50 - 1/100. Images of positive IHC with this monoclonal antibody, clone PEG-B-47b, are on the ab51257 datasheet.
Sandwich ELISA		Use at an assay dependent concentration.
WB		1/50000. Predicted molecular weight: 2 kDa. For unpurified use at 1/5000 - 1/20000.
ELISA		Use a concentration of 1 µg/ml. Coating plates for ELISA: 0 to 500 ng/ml. For unpurified use at 0.5µg/ml, coating plates for ELISA: 5 to 10 ug/ml.

别名
- PEG antibody

ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) (ab53449)

ELISA assay using 5 µg/mL Anti-AFP RabMAb as the capture antibody and 0.5 µg/mL Anti-PEG-47-Biotin RabMAb (unpurified ab53449) as the detection antibody. HRP-avidin (1/3000) used for anti-PEG-47 detection. This ELISA assay could detect YCA1017 peptide-PEG antigen at a concentration as low as 0.9 ng/mL.
ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) (ab53449)

Direct ELISA assay using 63 ng/mL of antigen (GCSF-PEG) to coat the plate. HRP-avidin (1/3000) was used for the detection of varying amounts of anti-PEG-47-Biotin (unpurified ab53449).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) (ab53449)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue showing negative staining with purified ab53449 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Western blot - Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) (ab53449)

Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) (ab53449) at 1/50000 dilution (purified) + BSA-PEG5K at 0.02 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

Predicted band size: 2 kDa
Observed band size: 280 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) (ab53449)

Lane 1 : Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) (ab53449) at 1/5000 dilution (unpurified)
Lane 2 : Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) (ab53449) at 1/10000 dilution (unpurified)
Lane 3 : Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) (ab53449) at 1/20000 dilution (unpurified)

All lanes : BSA-PEG

Predicted band size: 2 kDa
ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) (ab53449)

Direct ELISA antigen dose-response curve using purified ab53449. Antigen concentration of 0-500 ng/mL. A peroxidase-conjugated steptavidin (1/1000) was used.
Sandwich ELISA - Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) (ab53449)

Sandwich ELISA antigen dose-response curve using purified ab53449.

Capture antibody - Mouse anti-IFN alpha 2b (4000 ng/ml).

Antigens - PEG-IFN alpha 2b (0-1000 ng/ml).

Detection - ab53449 (500 ng) anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin) followed by peroxidase-conjugated streptravidin (1/1000).

This product has been referenced in:

Poirier N et al. FR104, an antagonist anti-CD28 monovalent fab' antibody, prevents alloimmunization and allows calcineurin inhibitor minimization in nonhuman primate renal allograft. Am J Transplant 15:88-100 (2015). Read more (PubMed: 25488654) »
der Valk FM et al. Prednisolone-containing liposomes accumulate in human atherosclerotic macrophages upon intravenous administration. Nanomedicine N/A:N/A (2015). Read more (PubMed: 25791806) »

See all 10 Publications for this product

发表研究结果有使用 ab53449？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

Show All 评价 Q&A

提交评价提交问题

1-6 of 6 Abreviews or Q&A

Question

What size of the branched and linear PEG chain does this antibody react to. Will it react to atleast 500 kDa chain?

Abcam community

Verified customer

Asked on Feb 22 2013

Answer

The smallest PEG molecule which our antibody can detect is PEG750. Based on customer feedback, the largest PEG molecule detected is PEG 40K. Additionally, ab53449 detects both linear and branched PEG based on our in-house ELISA testing.

Abcam Scientific Support

回复于 Feb 22 2013

Application

ELISA

Sample

Human Serum (Processed blood sample.)

Specification

Processed blood sample.

Type

Sandwich (Detection)

Blocking step

BSA, Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Nov 20 2014

Question

Is ab53449 purified?

Abcam community

Verified customer

Asked on Aug 25 2014

Answer

Yes, ab53449 is purified before biotinylation. Protein A was used to purify this antibody.

Elena Valued Customer

Abcam Scientific Support

回复于 Aug 25 2014

Question

What is the difference between ab51257 and ab53449?

Abcam community

Verified customer

Asked on Aug 25 2014

Answer

Both antibodies ab51257 and ab53449 are the same clone (PEG-B-47). Product ab53449 is the biotinlyated version of ab51257.

Elena Valued Customer

Abcam Scientific Support

回复于 Aug 25 2014

Question

Which PEG antibody do you recommend in detecting linear PEG? When detecting branched PEG?

Abcam community

Verified customer

Asked on Aug 25 2014

Answer

Both ab51257 and ab53449 detect linear and branched PEG (based on our in-house ELISA results).

Elena Valued Customer

Abcam Scientific Support

回复于 Aug 25 2014

Question

I found in your catalog the following antibodies: ab73909 and ab53449, which I would like to have. Could you please tell me how much these antibodies cost, how long will it take to deliver them? Is it possible to make them without glycerol, BSA and sodium azide?
Best regards

Abcam community

Verified customer

Asked on Feb 20 2013

Answer

Thank you for your email.

Could you let me know how many glycerol, BSA and sodium azide free vials you would like to order? Once we have this information we can then confirm the supply.

Thank you very much for your cooperation. I will look forward to hearing from you soon.

Abcam Scientific Support

回复于 Feb 20 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

定制方案及合作关系

Anti-Polyethylene glycol抗体[PEG-B-47b] (Biotin) (ab53449)

概述

性能

相关产品

应用

靶标

图片

数据表及文件

文献

This product has been referenced in:

客户评价及客户问答

ELISA abreview for Anti-Polyethylene glycol antibody [PEG-B-47b] (Biotin)