Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Macrophages)
Permeabilization
Yes - 0.3% Triton X-100
Specification
Macrophages
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Same as blocking buffer
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: FITC Donkey Anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
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提交于 Nov 08 2016