Anti-Podoplanin抗体[RTD4E10] - BSA and Azide free (ab11936)
Key features and details
- Syrian hamster monoclonal [RTD4E10] to Podoplanin - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG1
概述
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产品名称
Anti-Podoplanin抗体[RTD4E10] - BSA and Azide free
参阅全部 Podoplanin 一抗 -
描述
叙利亚仓鼠单克隆抗体[RTD4E10] to Podoplanin - BSA and Azide free -
宿主
Syrian hamster -
特异性
The monoclonal antibody will detect podoplanin on the surface of mouse lymphatic endothelial cells and some epithelial cell types by immunostaining or immunohistochemistry. This antibody is designed to detect podoplanin, which is also referred to as gp38 (mouse and rat) and gp36 (human). -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Mouse
不与反应: Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Mouse normal skin FFPE tissue sections (IHC-P)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from cell culture supernatant. -
克隆
单克隆 -
克隆编号
RTD4E10 -
同种型
IgG1 -
研究领域
相关产品
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Conjugation kits
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11936于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (6) |
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. -
组织特异性
Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. -
序列相似性
Belongs to the podoplanin family. -
翻译后修饰
Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
The N-terminus is blocked. -
细胞定位
Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles. - Information by UniProt
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数据库链接
- Entrez Gene: 14726 Mouse
- SwissProt: Q62011 Mouse
- Unigene: 2976 Mouse
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别名
- Aggrus antibody
- D2-40 antibody
- Glycoprotein 36 KD antibody
see all
图片
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IHC image of Podoplanin staining in a section of formalin-fixed paraffin-embedded normal mouse skin. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6). Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab11936 at 1/250. The secondary antibody (shown in green) was ab180063 at 1/500 (Goat Anti-Syrian hamster IgG H&L (Alexa Fluor® 488) and counterstained using ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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ab11936 staining Podoplanin in murine skin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in paraformaldehyde and an enzymatic antigen retrieval step was performed using Protein K. Samples were then blocked with 5% serum for 30 minutes at room temperature and then incubated with ab11936 at a 1/400 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated goat anti-hamster IgG used at a 1/500 dilution.
数据表及文件
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Datasheet download
文献 (81)
ab11936 被引用在 81 文献中.
- Sastourné-Arrey Q et al. Adipose tissue is a source of regenerative cells that augment the repair of skeletal muscle after injury. Nat Commun 14:80 (2023). PubMed: 36604419
- Fischer A et al. Neutrophils direct preexisting matrix to initiate repair in damaged tissues. Nat Immunol 23:518-531 (2022). PubMed: 35354953
- Fleig S et al. Loss of vascular endothelial notch signaling promotes spontaneous formation of tertiary lymphoid structures. Nat Commun 13:2022 (2022). PubMed: 35440634
- Ciccimarra R et al. The normal and fibrotic mouse lung classified by spatial proteomic analysis. Sci Rep 12:8742 (2022). PubMed: 35610327
- Kataru RP et al. Structural and Functional Changes in Aged Skin Lymphatic Vessels. Front Aging 3:864860 (2022). PubMed: 35821848