Anti-PLK4抗体(ab2642)
Key features and details
- Goat polyclonal to PLK4
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PLK4抗体
参阅全部 PLK4 一抗 -
描述
山羊多克隆抗体to PLK4 -
宿主
Goat -
Tested Applications & Species
Application Species Flow Cyt HumanICC/IF HumanWB Human -
免疫原
Synthetic peptide:
ATCIGEKIEDFKVGN
, corresponding to N terminal amino acids 2-16 of Human PLK4. -
阳性对照
- Human colon lysate
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常规说明
GenBank Accession Number - NP_055079The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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Flow Cyt |
Human
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ICC/IF |
Human
|
WB |
Human
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All applications |
Mouse
Cow
Dog
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应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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Flow Cyt |
Use a concentration of 10 µg/ml.
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|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 109 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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ICC/IF
Use a concentration of 10 µg/ml. |
Flow Cyt
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 109 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 1 POLO box domain.
Contains 1 protein kinase domain. -
翻译后修饰
Ubiquitinated; leading to its degradation by the proteasome.
Tyrosine-phosphorylated by TEC. -
细胞定位
Cytoplasm > cytoskeleton > centrosome > centriole. Nucleus > nucleolus. Cleavage furrow. Associates with centrioles throughout the cell cycle. According to PubMed:16244668, it is not present at cleavage furrows. - Information by UniProt
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数据库链接
- Entrez Gene: 10733 Human
- Entrez Gene: 20873 Mouse
- SwissProt: O00444 Human
- SwissProt: Q64702 Mouse
- Unigene: 172052 Human
- Unigene: 3794 Mouse
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别名
- PLK 4 antibody
- PLK-4 antibody
- PLK4 antibody
see all
图片
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Immunofluorescence analysis of paraformaldehyde fixed HeLa cells staining PLK4. Cells were permeabilized with 0.15% Triton. Samples were incubated with primary anitbody for 1 hour at 10µg/ml. An Alexa Fluor 488 was used as the secondary antibody. DAPI was used as a nuclear counterstain. Unimmunized goat IgG (10µg/ml) was used as the negative control.
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Anti-PLK4 antibody (ab2642) at 2 µg/ml + human colon lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 109 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour -
Flow Cytometry analysis of HeLa cells labeling PLK4 with ab2642 at 10ug/mL followed by Alexa Fluor 488 secondary antibody (1ug/mL) (blue line). Cells were permeabilized with 0.5% Triton. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunofluorescence analysis of paraformaldehyde fixed A431 cells staining PLK4. Cells were permeabilized with 0.15% Triton. Samples were incubated with primary anitbody for 1 hour at 10µg/ml. An Alexa Fluor 488 was used as the secondary antibody. DAPI was used as a nuclear counterstain. Unimmunized goat IgG (10µg/ml) was used as the negative control.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab2642 被引用在 7 文献中.
- Shi J et al. Long non-coding RNA RUNX1-IT1 plays a tumour-suppressive role in colorectal cancer by inhibiting cell proliferation and migration. Cell Biochem Funct 37:11-20 (2019). PubMed: 30499136
- Zhang Z et al. PLK4 is a determinant of temozolomide sensitivity through phosphorylation of IKBKE in glioblastoma. Cancer Lett 443:91-107 (2019). PubMed: 30529153
- Czub B et al. TOPORS, a Dual E3 Ubiquitin and Sumo1 Ligase, Interacts with 26 S Protease Regulatory Subunit 4, Encoded by the PSMC1 Gene. PLoS One 11:e0148678 (2016). PubMed: 26872363
- Rosario CO et al. A novel role for Plk4 in regulating cell spreading and motility. Oncogene N/A:N/A (2014). PubMed: 25174401
- Rosario CO et al. Plk4 is required for cytokinesis and maintenance of chromosomal stability. Proc Natl Acad Sci U S A 107:6888-93 (2010). PubMed: 20348415
- Martindill DM et al. Nucleolar release of Hand1 acts as a molecular switch to determine cell fate. Nat Cell Biol 9:1131-41 (2007). WB, ICC/IF ; Rat . PubMed: 17891141
- Syed N et al. Transcriptional silencing of Polo-like Kinase 2 (Snk/Plk2) is a frequent event in B cell malignancies. Blood : (2005). PubMed: 16160013