Plasma膜蛋白Extraction试剂盒(ab65400)
Key features and details
- Assay time: 1 hr
概述
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产品名称
Plasma膜蛋白Extraction试剂盒 -
检测时间
1h 00m -
产品概述
Plasma Membrane Protein Extraction Kit ab65400 provides optimized buffers and reagents for effective extraction of plasma membrane proteins from mammalian tissues and cells.
Other commercially available methods can only extract the total cellular membrane proteins (combinations of plasma and organelle membrane proteins). The ab65400 kit offers two options: either extract the total cellular membrane proteins, or purify the plasma membrane proteins specifically.
The procedure offers consistent yield and high purity (over 90%). Membrane proteins prepared using the kit can be utilized in a variety of applications, such as Western blotting, 2-D gels, and enzyme analyses, etc. The entire procedure takes less than 1 hour.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K268 Membrane Protein Extraction Kit. K268-50 is the same size as the 50 test size of ab65400.
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 50 tests Homogenize Buffer NM 1 x 100ml Lower Phase Solution WM 1 x 20ml Protease Inhibitor Cocktail (Lyophilised) Red 1 vial Upper Phase Solution NM 1 x 20ml -
研究领域
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相关性
Membrane Protein Extraction is a method for extraction of membrane proteins from mammalian tissues and cells. Many procedures extract total cellular membrane proteins which is a combination of plasma and organelle membrane proteins.
图片
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Functional Studies - Plasma Membrane Protein Extraction Kit (ab65400)Kweon, Hae-Jin et al., Scientific reports?vol. 6 30684., Fig 1, doi:10.1038/srep30684Western blotting on the plasma membrane (PM) fraction extracted useing ab65400, total cellular membrane (TCM) fraction, and total lysate of cells expressing GFP-tagged ASIC2a or ASIC2b was performed using anti-GFP antibody. As controls, the PM and the TCM fractions were blotted using anti-calnexin (CNX) antibody, and total lysate was blotted using anti-GAPDH antibody.
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Membrane protein assay - ab65400Image from Choveau F.S et al., PLoS One 10(12), Fig 2C. Doi: 10.1371/journal.pone.0145367. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Cell membrane protein was collected using plasma membrane protein extraction kit (ab65400). The upper panels show the plasma membrane myc-tagged protein; the middle panels show the cytosolic myc-tagged proteins and the lower panels show the total myc-tagged protein from CHO (Chinese Hamster ovary) whole-cell lysates.
数据表及文件
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SDS download
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Datasheet download
文献 (110)
ab65400 被引用在 110 文献中.
- Chen Z et al. Cell surface GRP78 regulates BACE2 via lysosome-dependent manner to maintain mesenchymal phenotype of glioma stem cells. J Exp Clin Cancer Res 40:20 (2021). PubMed: 33413577
- Lin CN et al. An aptamer interacting with heat shock protein 70 shows therapeutic effects and prognostic ability in serous ovarian cancer. Mol Ther Nucleic Acids 23:757-768 (2021). PubMed: 33614227
- Miyata T et al. Differential role of MLKL in alcohol-associated and non-alcohol-associated fatty liver diseases in mice and humans. JCI Insight 6:N/A (2021). PubMed: 33616081
- Sadhukhan T et al. In a mouse model of INCL reduced S-palmitoylation of cytosolic thioesterase APT1 contributes to microglia proliferation and neuroinflammation. J Inherit Metab Dis 44:1051-1069 (2021). PubMed: 33739454
- Bhandari P et al. GABAB receptor auxiliary subunits modulate Cav2.3-mediated release from medial habenula terminals. Elife 10:N/A (2021). PubMed: 33913808