Anti-Placental alkaline phosphatase (PLAP)抗体[8B6] (ab33)

Thank you for your enquiry. Unfortunately we have not tested ab33 against mouse so we do not know whether it is suitable. The antibody was raised against the whole protein, and although the mouse and human protein show 75% homology we do not know which part of the protein it is reactive against and thus the exact homology in mouse. We do stock another antibody which is reactive in IHC-P on mouse samples, which would be more suitable: ab16695 - Anti-Placental alkaline phosphatase (PLAP) antibody [SP15] IHC-P, WB Reacts with Mouse and Human It has not been tested in ICC on primary cultures, but if you are interested in testing it as part of our https://www.abcam.com/abtrial program, just let me know. I hope this information helps.

Thank you for your enquiry. According to the database SwissProt the MW of the precursor PLAP is 57kDa and it is glycosylated therefore its weight should be higher than this. A paper by Webb et al (Placenta. 1986 Sep-Oct;7(5):405-15.Isolation of placental-type alkaline phosphatase associated with human syncytiotrophoblast membranes using monoclonal antibodies) describes "an eluted enzymatically active PLAP had a molecular weight in the regions of 115K and 130K, with an occasional higher molecular weight form at 180K. On reduction to a 66K monomeric form". Regarding dilutions to try, I would recommend trying a range of dilutions in a preliminary experiment (1:250, 1:500, 1:1000) with overnight incubation and optimising following the results of that experiment. If the customer decides to go ahead and purchase this product, please ask him/her to let us know how he/she gets on by submitting an Abreview and in return we will award him/her 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). Please let me know if you require further information,

I'm afraid that we do not have any data regarding the suitablity of this antibody for WB.

This antibody has not been tested in flow cytometry.

This antibody has not been tested in immunofluorescence.

This monoclonal was raised against the whole protein, and in the absence of any epitope mapping studies, I am afraid that we do not know against which part of the protein the antibody is reactive.

Vlad We do not have any data on this antibody's inhibiting activity in vivo or in vitro. We are about to implement a trial 'try risk free' scheme which says that if an antibody is purchased and used in an experiment outside that written on the datasheet, and it is found not to work, in return for the negative data (which we can add to the datasheet to help other researchers) we will refund or issue a credit note.

This antibody has not yet been tested in Western blotting.