Anti-PKM抗体- N-terminal (ab196560)
Key features and details
- Rabbit polyclonal to PKM - N-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-PKM抗体- N-terminal
参阅全部 PKM 一抗 -
描述
兔多克隆抗体to PKM - N-terminal -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human PKM2 (N terminal).
Database link: P14618 -
阳性对照
- Jurkat, HeLa and A549 extracts. Human, mouse and rat lung cancer tissue. HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab196560于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/20 - 1/100.
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IHC-P |
1/50 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 58 kDa.
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说明 |
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ICC/IF
1/20 - 1/100. |
IHC-P
1/50 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 58 kDa. |
靶标
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功能
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. -
组织特异性
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
序列相似性
Belongs to the pyruvate kinase family. -
翻译后修饰
ISGylated.
Under hypoxia, hydroxylated by EGLN3.
Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.
FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35. -
细胞定位
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity. - Information by UniProt
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数据库链接
- Entrez Gene: 5315 Human
- SwissProt: P14618 Human
- Unigene: 534770 Human
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别名
- CTHBP antibody
- Cytosolic thyroid hormone-binding protein antibody
- KPYM_HUMAN antibody
see all
图片
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All lanes : Anti-PKM antibody - N-terminal (ab196560) at 1/500 dilution
Lane 1 : Jurkat extracts
Lane 2 : HeLa extracts
Lane 3 : A549 extracts
Predicted band size: 58 kDa -
Immunohistochemical analysis of paraffin-embedded human lung tissue labeling PKM2 with ab196560 at 1/50 dilution.
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Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling PKM2 with ab196560 at 1/100 dilution.
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Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling PKM2 with ab196560 at 1/100 dilution.
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Immunofluorescence analysis of HeLa cells labeling PKM2 using ab196560, at 1/20 dilution. Lower panel shows DAPI for nuclear staining (Blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab196560 尚未被引用在任何文献中。