Anti-PKC zeta抗体(ab4137)

Submit a review Q&A (3)References (3)

Key features and details

  • Rabbit polyclonal to PKC zeta
  • Suitable for: WB, ELISA, IHC-Fr, ICC
  • Reacts with: Rat
  • Isotype: IgG

概述

  • 产品名称

    Anti-PKC zeta抗体
    参阅全部 PKC zeta 一抗

  • 描述

    兔多克隆抗体to PKC zeta

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, ELISA, IHC-Fr, ICCmore details

  • 种属反应性

    与反应: Rat
    预测可用于: Mammals

  • 免疫原

    Synthetic peptide:

    EYINPLLLSAEESV

    , corresponding to amino acids 579-592 of Rat PKC zeta.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.05% Sodium azide
  • Concentration information loading...

  • 纯度

    Whole antiserum

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Predicted molecular weight: 71 kDa. (using horseradish peroxidase or alkaline phosphatase).
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC 1/250. Frozen. (using horseradish peroxidase or alkaline phosphatase).

靶标

  • 功能

    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.

  • 组织特异性

    Expressed in brain, and to a lesser extent in lung, kidney and testis.

  • 序列相似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 OPR domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.

  • 结构域

    The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
    The C1 domain does not bind the diacylglycerol (DAG).

  • 翻译后修饰

    CDH5 is required for its phosphorylation at Thr-410.

  • 细胞定位

    Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 14-3-3-zetaisoform antibody
      • AI098070 antibody
      • aPKCzeta antibody
      • C80388 antibody
      • EC 2.7.11.13 antibody
      • KPCZ_HUMAN antibody
      • nPKC zeta antibody
      • nPKC-zeta antibody
      • OTTHUMP00000001368 antibody
      • OTTHUMP00000044160 antibody
      • PKC 2 antibody
      • PKC ZETA antibody
      • PKC2 antibody
      • Pkcz antibody
      • PKCZETA antibody
      • PKM-zeta, included antibody
      • PRKCZ antibody
      • Protein kinase C zeta antibody
      • Protein kinase C zeta form antibody
      • Protein kinase C zeta type antibody
      • r14-3-3 antibody
      • R74924 antibody
      • zetaPKC antibody
      see all

    图片

    • Western blot - Anti-PKC zeta antibody (ab4137)
      Western blot - Anti-PKC zeta antibody (ab4137)Image from Barros SA et al, J Biol Chem. 2009 Jan 30;284(5):2672-9. Epub 2008 Nov 21, Fig 2.
      Immunoprecipitation.
      Rat renal cortex from adult male Sprague-Dawley CD rats was isolated and suspended in CelLyticTM MT Mammalian Tissue Lysis/Extraction reagent containing complete protease inhibitor mixture. Tissues were then homogenized and centrifuged at 2,500 x g for 10 minutes at 4 °C. The supernatant was used for immunoprecipitation. To eliminate nonspecific binding, 1 ml of rat kidney lysate (3 mg of protein) was pre-cleared by incubation with 50µl of TrueBlotTM anti-rabbit Ig IP beads overnight at 4 °C. Cleared cell lysates were incubated with either 7µg of ab4137 or 1µg of polyclonal rabbit anti-rOAT3 for 2 hours at 4 °C. TrueBlot anti-rabbit Ig IP beads (30µl) was added and incubation was continued for 2 hours at 4 °C. Bound proteins were washed 7 times with increasing washing stringency IP buffers and eluted from beads by heating in 4 x NuPAGE LDS sample buffer for 10 minutes. The Invitrogen NuPAGE Novex Tris

    文献 (3)

    发表研究结果有使用 ab4137？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab4137 被引用在 3 文献中.

    • Levy D  et al. Lysine methylation of the NF-?B subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-?B signaling. Nat Immunol 12:29-36 (2011). PubMed: 21131967
    • Barros SA  et al. Activation of protein kinase Czeta increases OAT1 (SLC22A6)- and OAT3 (SLC22A8)-mediated transport. J Biol Chem 284:2672-9 (2009). WB, IP ; Rat . PubMed: 19028678
    • Ort T  et al. Alterations in soleus muscle gene expression associated with a metabolic endpoint following exercise training by lean and obese Zucker rats. Physiol Genomics 29:302-11 (2007). PubMed: 17284668

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Question

    Dear Madam/Sir,   I have recently read an interesting article regarding the regulatory role of PKCzeta isoform on F0F1 ATP synthase beta subunit. It claimed that PKCzeta is able to phosphorilate the beta subunit of the F0F1 ATP synthase and hence, regulate its activity. The phosphorilation was shown by Western blot using antibodies raised against the phospho- and the nonphospho forms of the synthase. Unfortunately, it was not written who was the provider of the antibodies, and the authors didn't reply my e-mails to tell it to me.    Finally, I have checked the homepage of MitoSciences, but I failed to find antibodies against the phospho form of the ATP syntase, so my question is: could you provide me antibodies raised against both the phospho and the nonphospho forms of the beta subunit of human F0F1 ATP synthase? Or alternatively, could you suggest any methods with which I could measure somehow the activity of the ATP synthase?   Thank you in advance! Looking forward to your answer, I wish you all the best,    

    Read More
    Answer

    Thank you for your inquiry. I have conducted a search for you and found the following antibodies: ab43176 - Rabbit polyclonal to PKC zeta ab4137 - Chicken polyclonal to ATP Synthase beta ab51157 - Rabbit polyclonal to PKC zeta ab59412 - Rabbit polyclonal to PKC zeta (phospho T560) ab62372 - Rabbit monoclonal [EP2037AY] to PKC zeta (phospho T560)  We have a number of antibodies in our catalog that might be of interest to you. I would like to direct you to the search facility on our website (www.abcam.com). Please enter the name of the protein into the search box at the top of the home page. A list of products will be generated which can be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in. I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.              

    Read More

    Question

    BATCH NUMBER 119876 DESCRIPTION OF THE PROBLEM I see an obvious band at 50 KD instead of around 71 KD. It is not at right size. SAMPLE Human cell extract PRIMARY ANTIBODY PKC zeta antibody (abcam/ rabbit/1:250/overnight incubation/3 times of TBST for about 0.5 hour) DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED None ANTIBODY STORAGE CONDITIONS 4 degree SAMPLE PREPARATION EBC buffer plus complete min AMOUNT OF PROTEIN LOADED 30-50 ug ELECTROPHORESIS/GEL CONDITIONS 10% SDS gel with Biorad running buffer TRANSFER AND BLOCKING CONDITIONS Semidry transfer with Biorad system. 5% milk in TBST buffer SECONDARY ANTIBODY anti-rabbit IgG, Horseradish Peroxidase from [ a competitor] HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? once HAVE YOU RUN A "NO PRIMARY" CONTROL? No WHAT STEPS HAVE YOU ALTERED? No

    Read More
    Answer

    Thank you for your patience and for providing details regarding your protocol. Ab4137 was characterized for use in Western blotting with rat brain and myeloid tissues. This antibody has not yet been tested for cross-reactivity with human samples. I would therefore recommend using rat brain or myeloid tissue as a positive control. The antibody is predicted to react with other mammals due to sequence homology, but this hasn't been tested so we cannot guarantee results. The originator of this antibody also mentioned that the calculated molecular weight is 67.7 kDa but it is common to find that the protein resolves closer to 76-80 kDa. I know that you mentioned seeing a band at approximately 50 kDa, but try the antibody again with a positive control (also, make sure that there are sufficient protease inhibitors in your sample buffer). I hope this helps, please contact us again if you need additional assistance.

    Read More

    Question

    Customer is using this antibody in WB with rat samples. At a dilution of 1:250 and incubation overnight at 4C, there are no bands. Secondary is goat anti-rabbit at 1:1500 and is working with other primaries. Abcam lot# 105842.

    Read More
    Answer

    Thank you for your enquiry. Did you try increasing the concentration of the primary to 1:100? Please let me know if that improved your results. What specifically are the rat samples that you are using? Can you tell me as well, how did you store the antibody? Thanks again, and I look forward to hearing from you.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com