Key features and details
- Rabbit polyclonal to PKC theta/PRKCQ
- Suitable for: ELISA, IHC-P, IHC-Fr, WB
- Reacts with: Rat
- Isotype: IgG
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
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Our Abpromise guarantee covers the use of ab4133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry: 1/250 (alkaline phosphatase or horseradish peroxidase).
Western blot (enhanced chemiluminescence: 1/250 (alkaline phosphatase or horseradish peroxidase). Predicted molecular weight: 85 kDa.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
With a more sensitive detection system, such as
enhanced chemiluminescence or ELISA, the antibody may be diluted further.
It has also been reported to be useful for
immunoprecipitation of PKC theta.
功能This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
组织特异性Skeletal muscle, megakaryoblastic cells and platelets.
序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
结构域The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
翻译后修饰Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
- Information by UniProt
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody