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A synthetic phospho-peptide from residues surrounding Tyrosine 311 of human PKC delta.
A trial size is available to purchase for this antibody.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab76181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500 - 1/5000. Predicted molecular weight: 77 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Dot blot analysis of PKC delta (pY311) phospho peptide (Lane 1) and PKC delta non-phospho peptide (Lane 2) using ab76181 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.
Blocking and Diluting buffer and concentration: 5% NFDM /TBST.
Exposure time: 3 minutes.
ab76181 at 1/20 immunoprecipitating PKC delta (phospho Y311) in HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.
Lane 1 (input): HeLa treated with 10mM H2O2 for 1 hour whole cell lysate (10µg)
Lane 2 (+): ab76181 + HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76181 in HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.
For western blotting, ab76181 was used as a dilution of 1/200 followed by ab131366 VeriBlot for IP (HRP) at a dilution of 1/1000.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Blocking- 5% Milk for 1 hour at 25°C.
A non-specific band was observed at 80 kDa.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"