为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC

Anti-PKC抗体[MC5] (ab31)

  • Datasheet
  • SDS
Reviews (6)Q&A (4)References (26)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-PKC antibody [MC5] (ab31)
  • Flow Cytometry - Anti-PKC antibody [MC5] (ab31)

Key features and details

  • Mouse monoclonal [MC5] to PKC
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2a

你可能会感兴趣

二抗
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

查看更多相关产品

概述

  • 产品名称

    Anti-PKC抗体[MC5]
    参阅全部 PKC 一抗
  • 描述

    小鼠单克隆抗体[MC5] to PKC
  • 宿主

    Mouse
  • 特异性

    This antibody reacts with PKC alpha, crossreactivity to PKC beta isoformes can be observed.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • 免疫原

    Purified bovine brain protein kinase C

  • 阳性对照

    • WB: Calpain treated PKC. Rat adipocyte homogenate. 3T3-L1 homogenate. Purified microsomes from 3T3-L1 cells. Flow Cytometry: Jurkat cells. ICC/IF: Vero and HEK-293T cells. IHC-Fr: mouse spleen and duodenum tissue. IHC-P: Human skin papilloma tissue.
  • 常规说明

    It should be possible to selectively block degradation of PKC without affecting the membrane associated activation. This will allow an assessment of the role of proteolysis in the activation of the protein kinase pathway.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    MC5
  • 骨髓瘤

    P3x63
  • 同种型

    IgG2a
  • 轻链类型

    unknown
  • 研究领域

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • Other
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab31 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
WB
Human
应用 Ab评论 说明
WB (2)
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

说明
WB
Use at an assay dependent concentration.
Flow Cyt
Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

靶标

  • 功能

    Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppressive form of RB1 and induction of the CDK inhibitors CDKN1A and CDKN1B. Exhibits anti-apoptotic function in glioma cells and protects them from apoptosis by suppressing the p53/TP53-mediated activation of IGFBP3, and in leukemia cells mediates anti-apoptotic action by phosphorylating BCL2. During macrophage differentiation induced by macrophage colony-stimulating factor (CSF1), is translocated to the nucleus and is associated with macrophage development. After wounding, translocates from focal contacts to lamellipodia and participates in the modulation of desmosomal adhesion. Plays a role in cell motility by phosphorylating CSPG4, which induces association of CSPG4 with extensive lamellipodia at the cell periphery and polarization of the cell accompanied by increases in cell motility. Is highly expressed in a number of cancer cells where it can act as a tumor promoter and is implicated in malignant phenotypes of several tumors such as gliomas and breast cancers. Negatively regulates myocardial contractility and positively regulates angiogenesis, platelet aggregation and thrombus formation in arteries. Mediates hypertrophic growth of neonatal cardiomyocytes, in part through a MAPK1/3 (ERK1/2)-dependent signaling pathway, and upon PMA treatment, is required to induce cardiomyocyte hypertrophy up to heart failure and death, by increasing protein synthesis, protein-DNA ratio and cell surface area. Regulates cardiomyocyte function by phosphorylating cardiac troponin T (TNNT2/CTNT), which induces significant reduction in actomyosin ATPase activity, myofilament calcium sensitivity and myocardial contractility. In angiogenesis, is required for full endothelial cell migration, adhesion to vitronectin (VTN), and vascular endothelial growth factor A (VEGFA)-dependent regulation of kinase activation and vascular tube formation. Involved in the stabilization of VEGFA mRNA at post-transcriptional level and mediates VEGFA-induced cell proliferation. In the regulation of calcium-induced platelet aggregation, mediates signals from the CD36/GP4 receptor for granule release, and activates the integrin heterodimer ITGA2B-ITGB3 through the RAP1GAP pathway for adhesion. During response to lipopolysaccharides (LPS), may regulate selective LPS-induced macrophage functions involved in host defense and inflammation. But in some inflammatory responses, may negatively regulate NF-kappa-B-induced genes, through IL1A-dependent induction of NF-kappa-B inhibitor alpha (NFKBIA/IKBA). Upon stimulation with 12-O-tetradecanoylphorbol-13-acetate (TPA), phosphorylates EIF4G1, which modulates EIF4G1 binding to MKNK1 and may be involved in the regulation of EIF4E phosphorylation. Phosphorylates KIT, leading to inhibition of KIT activity. Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.
  • 序列相似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 细胞定位

    Cytoplasm. Cell membrane. Mitochondrion membrane. Nucleus.
  • Target information above from: UniProt accession P17252 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5578 Human
    • Entrez Gene: 5579 Human
    • Entrez Gene: 5580 Human
    • Entrez Gene: 5581 Human
    • Entrez Gene: 5582 Human
    • Entrez Gene: 5590 Human
    • Entrez Gene: 18750 Mouse
    • Entrez Gene: 18751 Mouse
    • Entrez Gene: 18752 Mouse
    • Entrez Gene: 18753 Mouse
    • Entrez Gene: 18754 Mouse
    • Entrez Gene: 18762 Mouse
    • Entrez Gene: 170538 Rat
    • Entrez Gene: 24680 Rat
    • Entrez Gene: 24681 Rat
    • Entrez Gene: 25023 Rat
    • Entrez Gene: 25522 Rat
    • Entrez Gene: 29340 Rat
    • Omim: 176970 Human
    • Omim: 176982 Human
    • SwissProt: P05129 Human
    • SwissProt: P05771 Human
    • SwissProt: P17252 Human
    • SwissProt: Q02156 Human
    • SwissProt: Q05513 Human
    • SwissProt: Q05655 Human
    • SwissProt: P20444 Mouse
    • SwissProt: P23298 Mouse
    • SwissProt: P63318 Mouse
    • SwissProt: P68404 Mouse
    • SwissProt: Q02956 Mouse
    • SwissProt: P05696 Rat
    • SwissProt: P09217 Rat
    • SwissProt: P63319 Rat
    • SwissProt: P68403 Rat
    • Unigene: 460355 Human
    • Unigene: 531704 Human
    • Unigene: 708867 Human
    • Unigene: 207496 Mouse
    • Unigene: 222178 Mouse
    • Unigene: 86669 Rat
    • Unigene: 91118 Rat
    see all
  • 别名

    • KPCA_HUMAN antibody
    • PKC alpha antibody
    • PKC beta antibody
    • PKC delta antibody
    • PKC epsilon antibody
    • PKC gamma antibody
    • PKC zeta antibody
    • PKC-A antibody
    • PKC-alpha antibody
    • PKC2 antibody
    • PKCA antibody
    • PKCB antibody
    • PKCD antibody
    • PKCE antibody
    • PKCG antibody
    • PRKCA antibody
    • PRKCB antibody
    • PRKCB1 antibody
    • PRKCB2 antibody
    • PRKCD antibody
    • PRKCE antibody
    • PRKCG antibody
    • PRKCZ antibody
    • Protein kinase C alpha antibody
    • Protein kinase C alpha type antibody
    • Protein kinase C antibody
    • Protein kinase C beta antibody
    • Protein kinase C delta antibody
    • Protein kinase C epsilon antibody
    • Protein kinase C gamma antibody
    • Protein kinase C zeta antibody
    see all

图片

  • Western blot - Anti-PKC antibody [MC5] (ab31)
    Western blot - Anti-PKC antibody [MC5] (ab31)
    All lanes : Anti-PKC antibody [MC5] (ab31)

    Lane 1 : Calpain treated PKC
    Lane 2 : Homogenate from rat adipocytes
    Lane 3 : Homogenate from 3T3-L1 cells
    Lane 4 : Purified microsomes from 3T3-L1 cells


    Review by Mikael Rutberg submitted 2 September 2004.

  • Flow Cytometry - Anti-PKC antibody [MC5] (ab31)
    Flow Cytometry - Anti-PKC antibody [MC5] (ab31)

    Overlay histogram showing Jurkat (Human T cell leukemia cell line from peripheral blood) cells stained with ab31 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab31, 1 μg/1x106 cells) for 30 minutes at 22°C. The secondary antibody used was a goat anti-mouse Alexa Fluor® 488 (IgG; H+L) ab150113 at 1/2000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1 μg/1x106 cells) used under the same conditions. Unlabeled sample (blue line) was used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This antibody gave a positive signal in HL-60 (Human promyelocytic leukemia cell line) cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.

实验方案

  • Flow cytometry protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (26)

    发表研究结果有使用 ab31?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab31 被引用在 26 文献中.

    • Xie X  et al. High glucose induced endothelial cell reactive oxygen species via OGG1/PKC/NADPH oxidase pathway. Life Sci 256:117886 (2020). PubMed: 32497631
    • Burger CA  et al. LKB1 coordinates neurite remodeling to drive synapse layer emergence in the outer retina. Elife 9:N/A (2020). PubMed: 32378514
    • Gu Z  et al. USP34 regulated human pancreatic cancer cell survival via AKT and PKC pathways. Biol Pharm Bull N/A:N/A (2019). PubMed: 30686807
    • Shaif NA  et al. Sasa Quelpaertensis Nakai Induced Antidepressant-Like Effect in Ovariectomized Rats. Biomed Res Int 2019:5815604 (2019). PubMed: 31380432
    • Hagiwara A  et al. Cytomatrix proteins CAST and ELKS regulate retinal photoreceptor development and maintenance. J Cell Biol 217:3993-4006 (2018). PubMed: 30190286
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 10 Abreviews or Q&A

    Question

    Do you have any information whether this antibody does recongize other isoforms of PKC? Can you please confirm which isoforms of PKC have been tested as well?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 18 2010

    Answer

    Indeed, this antibody is a clone and is used by many researchers. However I could not conclude on its specificity. Indeed, some sources quote that this antibody is specific for PKC alpha, whereas other state that there might be crossreactivity with PKC beta isoforms. In summary: we can guarantee that this antibody will recognize PKC alpha, we can however not exclude crossreactivity with PKC beta isoformes. Please do not hesitate to contact us should you have any questions or concerns.

    Read More

    Abcam Scientific Support

    回复于 Dec 10 2010

    Western blot abreview for Anti-PKC antibody [MC5]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human pancreatic cancer cell lines)
    Loading amount
    30 µg
    Specification
    human pancreatic cancer cell lines
    Gel Running Conditions
    Non-reduced Denaturing (7.5%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Pawel Mazur

    Verified customer

    提交于 Jul 31 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PKC antibody [MC5]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Skin papiloma)
    Specification
    Skin papiloma
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 15 2010

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PKC antibody [MC5]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse pancreatic neoplasia)
    Specification
    mouse pancreatic neoplasia
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Blocking step
    MOM kit blocking agaent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 14 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-PKC antibody [MC5]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (pancreatic tisse and tumor)
    Specification
    pancreatic tisse and tumor
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 09 2010

    Immunohistochemistry (Frozen sections) abreview for Anti-PKC antibody [MC5]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Mouse spleen and duodenum)
    Specification
    Mouse spleen and duodenum
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 09 2010

    Western blot abreview for Anti-PKC antibody [MC5]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Pancreatic cancer)
    Loading amount
    35 µg
    Specification
    Pancreatic cancer
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More

    Dr. Pawel Mazur

    Verified customer

    提交于 Jun 04 2010

    Question

    What type of tissue is recommended as a positive control for IHC-P?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 18 2006

    Answer

    Thank you for your enquiry. For ab4127, this antibody was validated on tPA induced myeloid cells for IHC. Also, melanocytes have been known to express PKC alpha and alternatively brain tissue is typically inherently expressive. For ab31, I regret to inform you that this antibody has not yet been tested for application in IHC. I have updated the online datasheet to reflect this, and apologize for this. Please contact us again if you have any additional questions or concerns.

    Read More

    Abcam Scientific Support

    回复于 Jan 20 2006

    Question

    Could you tell us which PKC isoform(s) would your PKC antibody [MC5] (ab31) recognize? We are interested in using immunofluorescence to determine the spatial distribution of PKC alpha. Do you suggest any other Ab?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 28 2005

    Answer

    I apologize for the delay in responding to your enquiry. The originator of this antibody attests that this antibody has been found to recognize Protein kinase Ca. Epitope: within the variable region V3 PKCa (the site of limited proteolysis on the native enzyme). If you require further information, please contact us again and we will gladly assist you.

    Read More

    Abcam Scientific Support

    回复于 May 11 2005

    Question

    any suggestions for a range of dilutions for Western blot?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 09 2002

    Answer

    We have no information about the dilutions originally used with this antibody. I would suggest trying the antibody at 1:1000, 1:250 and 1:2000 initially, the ideal dilution will depend on the details of your experiments.

    Read More

    Abcam Scientific Support

    回复于 Jul 11 2002

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有