Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Cell (5 um frozen retinal section)
Permeabilization
No
Specification
5 um frozen retinal section
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Serum, 0.5% Triton in PBS
Dilution
1/200
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H&L)
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight 550
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight 550
Additional data
Additional Notes
The gray in the image is DAPI-stained nuclei.
The experiment was immunofluorescence on frozen sections of zebrafish retina. Eye cups were fixed in paraformaldehyde overnight at 4 degrees C, and embedded in 20% sucrose/OCT prior to frozen sectioning.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Jenny Lenkowski
Verified customer
提交于 Aug 02 2017