PKA Kinase Activity Assay试剂盒(ab139435)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 4 hr 30 min
- Sample type: Adherent cells, Purified protein, Suspension cells, Tissue Extracts
概述
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产品名称
PKA Kinase Activity Assay试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sample < 10% 批次间 样品 n Mean SD CV% Sample <=12% -
样品类型
Adherent cells, Suspension cells, Tissue Extracts, Purified protein -
检测类型
Enzyme activity -
检测时间
4h 30m -
产品概述
PKA Kinase Activity Kit (ab139435) is a non-radioactive assay providing a safe, rapid and reliable method for the screening of inhibitors or activators of PKA and for quantitating the activity of PKA in purified or partially purified enzyme preparations. This kit is based on a solid phase enzyme-linked immuno-absorbent assay (ELISA) that utilizes a specific synthetic peptide as a substrate for PKA and a polyclonal antibody that recognizes the phosphorylated form of the substrate. The assay is designed for the analysis of PKA activity in the solution phase. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols.
The kit offers the following advantages:
1. Safe - non-radioactive measurement of kinase activity
2. Flexible - kinetic and end-point options available
3. Fast - results in < 4.5 hours
4. Efficient - only 30 µl diluted sample needed per well
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平台
Microplate reader
性能
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存放说明
Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 30ml Active PKA 20 ng/µl 1 x 30µl Antibody Dilution Buffer 1 x 10ml Anti-Rabbit IgG: HRP Conjugate 1 x 20µl ATP 1 x 2mg Kinase Assay Dilution Buffer 1 x 10ml PKA Phosphospecific Substrate Antibody 1 x 5ml PKA Substrate Microtiter Plate 1 unit Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml -
研究领域
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相关性
In cell biology, Protein kinase A (PKA) is a family of enzymes whose activity is dependent on cellular levels of cyclic AMP (cAMP). PKA is also known as cAMP-dependent protein kinase. Protein kinase A has several functions in the cell, including regulation of glycogen, sugar, and lipid metabolism.
相关产品
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图片
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1e7 3T3-L1 cells were treated for 15 minutes with 1 μM dexamethasone (ab120743) and 1 μg/mL insulin (ab123768) with the addition of 50 μM forskolin (ab120058), 0.5 mM IBMX (ab120840) or 0.1 mM 8-CPT-cAMP (ab120424). Cell extracts were prepared according to the protocol. Lysates were diluted 50-fold, and tested in duplicates (+/-SD).
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Titration of positive control (active PKA), background signal subtracted (duplicates; +/- SD).
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Titration of cAMP Protein Kinase (ab56268), background signal subtracted (duplicates; +/- SD).
数据表及文件
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SDS download
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Datasheet download
文献 (53)
ab139435 被引用在 53 文献中.
- Fan T et al. Phosphodiesterase 4D promotes angiotensin II-induced hypertension in mice via smooth muscle cell contraction. Commun Biol 5:81 (2022). PubMed: 35058564
- Chen A et al. mTORC1 induces plasma membrane depolarization and promotes preosteoblast senescence by regulating the sodium channel Scn1a. Bone Res 10:25 (2022). PubMed: 35256591
- Li S et al. Impaired oxygen-sensitive regulation of mitochondrial biogenesis within the von Hippel-Lindau syndrome. Nat Metab 4:739-758 (2022). PubMed: 35760869
- Wan L et al. GP73 is a glucogenic hormone contributing to SARS-CoV-2-induced hyperglycemia. Nat Metab 4:29-43 (2022). PubMed: 34992299
- Lacalle E et al. Bicarbonate-Triggered In Vitro Capacitation of Boar Spermatozoa Conveys an Increased Relative Abundance of the Canonical Transient Receptor Potential Cation (TRPC) Channels 3, 4, 6 and 7 and of CatSper-γ Subunit mRNA Transcripts. Animals (Basel) 12:N/A (2022). PubMed: 35454259