Anti-PKA alpha + beta (catalytic subunits) (phospho T197)抗体(ab5815)
Key features and details
- Rabbit polyclonal to PKA alpha + beta (catalytic subunits) (phospho T197)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-PKA alpha + beta (catalytic subunits) (phospho T197)抗体
参阅全部 PKA alpha + beta (catalytic subunits) 一抗 -
描述
兔多克隆抗体to PKA alpha + beta (catalytic subunits) (phospho T197) -
宿主
Rabbit -
特异性
This antibody exibited a preference for PKA catalytic subunit beta in some tested cell lines. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Cow, Pig
-
免疫原
Synthetic peptide corresponding to PKA alpha + beta (catalytic subunits) (phospho T197).
-
阳性对照
- Forskolin-treated NIH3T3 cells, and Y-1 mouse adrenal cortical cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKA. The final product is generated by affinity chromatography using a PKA-derived peptide that is phosphorylated at threonine 197. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.75 µg/ml. Detects a band of approximately 42 kDa.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 0.75 µg/ml. Detects a band of approximately 42 kDa. |
靶标
-
相关性
PRKACA and PRKACB are members of the Ser/Thr protein kinase family and are a catalytic subunit of cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. -
细胞定位
Cytoplasm. Nucleus. Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm -
数据库链接
- Entrez Gene: 282322 Cow
- Entrez Gene: 282323 Cow
- Entrez Gene: 18747 Mouse
- Entrez Gene: 18749 Mouse
- SwissProt: P00517 Cow
- SwissProt: P05132 Mouse
- SwissProt: P05206 Mouse
-
别名
- cAMP dependent protein kinase beta catalytic subunit antibody
- cAMP dependent protein kinase alpha catalytic subunit antibody
- cAMP dependent protein kinase catalytic subunit alpha antibody
see all
图片
-
Western blot - Anti-PKA alpha + beta (catalytic subunits) (phospho T197) antibody (ab5815)Peptide Competition and Phosphatase Treatment: Lysates prepared from Y1 Adrenocortical cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% BSA-TBST buffer for two hours at room temperature, then incubated with 0.35
µ g/mL ab5815 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the peptide corresponding to PKA [pT197] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
Peptide Competition and Phosphatase Treatment: Lysates prepared from Y1 Adrenocortical cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% BSA-TBST buffer for two hours at room temperature, then incubated with 0.35 µg/mL ab5815 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the peptide corresponding to PKA [pT197] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
数据表及文件
-
Datasheet download
文献 (9)
ab5815 被引用在 9 文献中.
- Kurowska P et al. Role of vaspin in porcine ovary: effect on signaling pathways and steroid synthesis via GRP78 receptor and protein kinase A†. Biol Reprod 102:1290-1305 (2020). PubMed: 32149334
- Dawid M et al. Apelin decreased placental hormone secretion by human trophoblast BeWo cells via apelin receptor, protein kinase A and extracellular signal-regulated kinases 1/2 activation. J Physiol Pharmacol 70:N/A (2019). PubMed: 32084650
- Chen Z & Xue C G-Protein-Coupled Receptor 5 (LGR5) Overexpression Activates ß-Catenin Signaling in Breast Cancer Cells via Protein Kinase A. Med Sci Monit Basic Res 25:15-25 (2019). PubMed: 30662060
- Su P et al. Disruption of SynGAP-dopamine D1 receptor complexes alters actin and microtubule dynamics and impairs GABAergic interneuron migration. Sci Signal 12:N/A (2019). PubMed: 31387938
- Krag TO et al. Differential glucose metabolism in mice and humans affected by McArdle disease. Am J Physiol Regul Integr Comp Physiol 311:R307-14 (2016). PubMed: 27280431
- Jiang Z et al. Blocking mammalian target of rapamycin alleviates bone cancer pain and morphine tolerance via µ-opioid receptor. Int J Cancer 138:2013-20 (2016). WB ; Rat . PubMed: 26566757
- Bao Y et al. Topical treatment with Xiaozheng Zhitong Paste alleviates bone cancer pain by inhibiting proteinase-activated receptor 2 signaling pathway. Oncol Rep 34:1449-59 (2015). PubMed: 26133236
- Panas MW et al. Trace amine associated receptor 1 signaling in activated lymphocytes. J Neuroimmune Pharmacol 7:866-76 (2012). Human . PubMed: 22038157
- Ellison GM et al. Acute beta-adrenergic overload produces myocyte damage through calcium leakage from the ryanodine receptor 2 but spares cardiac stem cells. J Biol Chem 282:11397-409 (2007). PubMed: 17237229
