Anti-PKA alpha + beta (catalytic subunits) (phospho T197)抗体(ab5815)
Key features and details
- Rabbit polyclonal to PKA alpha + beta (catalytic subunits) (phospho T197)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-PKA alpha + beta (catalytic subunits) (phospho T197)抗体
参阅全部 PKA alpha + beta (catalytic subunits) 一抗 -
描述
兔多克隆抗体to PKA alpha + beta (catalytic subunits) (phospho T197) -
宿主
Rabbit -
特异性
This antibody exibited a preference for PKA catalytic subunit beta in some tested cell lines. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Cow, Pig -
免疫原
Synthetic peptide corresponding to PKA alpha + beta (catalytic subunits) (phospho T197).
-
阳性对照
- Forskolin-treated NIH3T3 cells, and Y-1 mouse adrenal cortical cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKA. The final product is generated by affinity chromatography using a PKA-derived peptide that is phosphorylated at threonine 197. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 0.1 - 0.75 µg/ml. Detects a band of approximately 42 kDa.
|
说明 |
---|
WB
Use a concentration of 0.1 - 0.75 µg/ml. Detects a band of approximately 42 kDa. |
靶标
-
相关性
PRKACA and PRKACB are members of the Ser/Thr protein kinase family and are a catalytic subunit of cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. -
细胞定位
Cytoplasm. Nucleus. Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm -
数据库链接
- Entrez Gene: 282322 Cow
- Entrez Gene: 282323 Cow
- Entrez Gene: 18747 Mouse
- Entrez Gene: 18749 Mouse
- SwissProt: P00517 Cow
- SwissProt: P05132 Mouse
- SwissProt: P05206 Mouse
-
别名
- cAMP dependent protein kinase beta catalytic subunit antibody
- cAMP dependent protein kinase alpha catalytic subunit antibody
- cAMP dependent protein kinase catalytic subunit alpha antibody
see all
图片
-
Peptide Competition and Phosphatase Treatment: Lysates prepared from Y1 Adrenocortical cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% BSA-TBST buffer for two hours at room temperature, then incubated with 0.35
µ g/mL ab5815 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the peptide corresponding to PKA [pT197] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
Peptide Competition and Phosphatase Treatment: Lysates prepared from Y1 Adrenocortical cells were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% BSA-TBST buffer for two hours at room temperature, then incubated with 0.35 µg/mL ab5815 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’ 2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the peptide corresponding to PKA [pT197] blocks the antibody signal, thereby demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.
数据表及文件
-
Datasheet download
文献 (12)
ab5815 被引用在 12 文献中.
- Mancinelli R et al. The Effects of Taurocholic Acid on Biliary Damage and Liver Fibrosis Are Mediated by Calcitonin-Gene-Related Peptide Signaling. Cells 11:N/A (2022). PubMed: 35563897
- Zhao J et al. Repeated exposure to sevoflurane in neonatal rats impairs cognition in adulthood via the PKA-CREB-BDNF signaling pathway. Exp Ther Med 22:1442 (2021). PubMed: 34721684
- Kurowska P et al. The role of vaspin in porcine corpus luteum. J Endocrinol 247:283-294 (2020). PubMed: 33108345
- Kurowska P et al. Role of vaspin in porcine ovary: effect on signaling pathways and steroid synthesis via GRP78 receptor and protein kinase A†. Biol Reprod 102:1290-1305 (2020). PubMed: 32149334
- Dawid M et al. Apelin decreased placental hormone secretion by human trophoblast BeWo cells via apelin receptor, protein kinase A and extracellular signal-regulated kinases 1/2 activation. J Physiol Pharmacol 70:N/A (2019). PubMed: 32084650