Anti-PKA alpha + beta (catalytic subunits)抗体(ab216572)
Key features and details
- Rabbit polyclonal to PKA alpha + beta (catalytic subunits)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PKA alpha + beta (catalytic subunits)抗体
参阅全部 PKA alpha + beta (catalytic subunits) 一抗 -
描述
兔多克隆抗体to PKA alpha + beta (catalytic subunits) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Synthetic peptide within Cow PKA alpha + beta (catalytic subunits) aa 1-100 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P05131 -
阳性对照
- Human lung carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab216572于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500.
|
|
IHC-P |
1/100 - 1/500.
|
说明 |
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WB
1/500. |
IHC-P
1/100 - 1/500. |
靶标
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相关性
PRKACA and PRKACB are members of the Ser/Thr protein kinase family and are a catalytic subunit of cAMP-dependent protein kinase. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. -
细胞定位
Cytoplasm. Nucleus. Note=Translocates into the nucleus (monomeric catalytic subunit). The inactive holoenzyme is found in the cytoplasm -
数据库链接
- Entrez Gene: 282322 Cow
- Entrez Gene: 282323 Cow
- Entrez Gene: 5566 Human
- Entrez Gene: 5567 Human
- Entrez Gene: 18747 Mouse
- Entrez Gene: 18749 Mouse
- Entrez Gene: 25636 Rat
- Omim: 176892 Human
see all -
别名
- cAMP dependent protein kinase beta catalytic subunit antibody
- cAMP dependent protein kinase alpha catalytic subunit antibody
- cAMP dependent protein kinase catalytic subunit alpha antibody
see all
图片
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Anti-PKA alpha + beta (catalytic subunits) antibody (ab216572) at 1/500 dilution + Jurkat lysates
Secondary
Conjugated secondary antibody at 1/10000 dilution -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling cAMP Protein Kinase Catalytic subunit with ab216572 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab216572 尚未被引用在任何文献中。