Anti-PIST抗体(ab37036)
Key features and details
- Rabbit polyclonal to PIST
- Suitable for: WB, IHC-P, ICC
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-PIST抗体
参阅全部 PIST 一抗 -
描述
兔多克隆抗体to PIST -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICCmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide of 16 amino acid from near the carboxy terminus of PIST (Human)
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阳性对照
- WB: Rat colon cell lysate. IHC-P: Rat colon tissue. ICC: Rat colon cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37036于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 51 kDa.
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IHC-P |
Use a concentration of 1 µg/ml.
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ICC |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 51 kDa. |
IHC-P
Use a concentration of 1 µg/ml. |
ICC
Use a concentration of 10 µg/ml. |
靶标
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功能
Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. -
组织特异性
Ubiquitously expressed. -
疾病相关
Note=A chromosomal aberration involving GOPC is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity. -
序列相似性
Contains 1 PDZ (DHR) domain. -
结构域
The PDZ domain mediates interactions with FZD5, FZD8, ACCN3, GRID2, CLCN3 (By similarity). Mediates also interaction with CFTR and ADRB1.
The coiled-coil region probably mediates association to membranes, targeting to the Golgi, and interactions with GOLGA3, and STX6. May also mediate interaction with RHOQ. -
细胞定位
Cytoplasm. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Enriched in synaptosomal and postsynaptic densities (PSD) fractions (By similarity). Expressed in cell bodies and dendrites of Purkinje cells (By similarity). Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides. - Information by UniProt
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数据库链接
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别名
- CAL antibody
- CFTR associated ligand antibody
- CFTR-associated ligand antibody
see all
图片
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Lane 1 : Anti-PIST antibody (ab37036) at 1 µg/ml
Lane 2 : Anti-PIST antibody (ab37036) at 2 µg/ml
Lane 3 : Anti-PIST antibody (ab37036) at 4 µg/ml
All lanes : rat colon cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 51 kDa -
ab37036 at 1 µg/ml staining PIST in rat colon tissue by IHC.
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Immunocytochemical analysis of PIST in Rat Colon cells using ab37036 at 10 µg/mL.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab37036 被引用在 7 文献中.
- Luo WY et al. PDZ Scaffold Protein CAL Couples with Metabotropic Glutamate Receptor 5 to Protect Against Cell Apoptosis and Is a Potential Target in the Treatment of Parkinson's Disease. Neurotherapeutics 16:761-783 (2019). PubMed: 31073978
- Li Y et al. A nonsense mutation in Ccdc62 gene is responsible for spermiogenesis defects and male infertility in repro29/repro29 mice. Biol Reprod 96:587-597 (2017). PubMed: 28339613
- Han F et al. Globozoospermia and lack of acrosome formation in GM130-deficient mice. Cell Death Dis 8:e2532 (2017). PubMed: 28055014
- Ohara N et al. Low expression of the GOPC is a poor prognostic marker in colorectal cancer. Oncol Lett 14:4483-4490 (2017). PubMed: 29085445
- Liu C et al. Sirt1 regulates acrosome biogenesis by modulating autophagic flux during spermiogenesis in mice. Development 144:441-451 (2017). PubMed: 28003215
- Wang H et al. Atg7 is required for acrosome biogenesis during spermatogenesis in mice. Cell Res 24:852-69 (2014). Mouse . PubMed: 24853953
- Xiao N et al. PICK1 deficiency causes male infertility in mice by disrupting acrosome formation. J Clin Invest 119:802-12 (2009). ICC/IF ; Rat, Mouse . PubMed: 19258705