重组Anti-PIP5K1C抗体[MAO-R1] (ab109192)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MAO-R1] to PIP5K1C
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PIP5K1C抗体[MAO-R1]
参阅全部 PIP5K1C 一抗 -
描述
兔单克隆抗体[MAO-R1] to PIP5K1C -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Full length protein corresponding to Mouse PIP5K1C.
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阳性对照
- WB: HeLa, Neuro-2a, K562, C6 and 293T cell lysates; Human fetal kidney tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
MAO-R1 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109192于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 80-90 kDa (predicted molecular weight: 73 kDa).
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 80-90 kDa (predicted molecular weight: 73 kDa). |
靶标
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功能
Plays a role in membrane ruffling and assembly of clathrin-coated pits at the synapse. Mediates RAC1-dependent reorganization of actin filaments (By similarity). Participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. -
疾病相关
Defects in PIP5K1C are the cause of lethal congenital contracture syndrome type 3 (LCCS3) [MIM:611369]; also known as multiple contractural syndrome Israeli Bedouin type B. LCCS is an autosomal recessive disorder characterized by early fetal hydrops and akinesia, the Pena-Shokeir phenotype, specific neuropathology with degeneration of anterior horn neurons and extreme skeletal muscle atrophy. LCCS3 patients present at birth with severe multiple joint contractures with severe muscle wasting and atrophy, mainly in the legs. LCCS3 can be distinguished from the original LCCS by the absence of hydrops, fractures, and multiple pterygia. -
序列相似性
Contains 1 PIPK domain. -
细胞定位
Cell membrane. Cytoplasmic, associated with the plasma membrane. Detected in focal adhesion plaques, membrane ruffles and plasma membrane invaginations. - Information by UniProt
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数据库链接
- Entrez Gene: 23396 Human
- Entrez Gene: 18717 Mouse
- Entrez Gene: 314641 Rat
- Omim: 606102 Human
- SwissProt: O60331 Human
- SwissProt: O70161 Mouse
- SwissProt: Q5I6B8 Rat
- Unigene: 282177 Human
see all -
别名
- Diphosphoinositide kinase antibody
- LCCS3 antibody
- Phosphatidylinositol-4-phosphate 5-kinase type I gamma antibody
see all
图片
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All lanes : Anti-PIP5K1C antibody [MAO-R1] (ab109192) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PIP5K1C knockout HeLa cell lysate
Lane 3 : Neuro-2a cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Predicted band size: 73 kDaLanes 1-4: Merged signal (red and green). Green - ab109192 observed at 73 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109192 Anti-PIP5K1C antibody [MAO-R1] was shown to specifically react with PIP5K1C in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265545 (knockout cell lysate ab258117) was used. Wild-type and PIP5K1C knockout samples were subjected to SDS-PAGE. ab109192 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-PIP5K1C antibody [MAO-R1] (ab109192) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Human fetal kidney cell lysate
Lane 3 : C6 cell lysate
Lane 4 : Neuro-2a cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 73 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab109192 被引用在 9 文献中.
- Yu M et al. Targeting type Iγ phosphatidylinositol phosphate kinase overcomes oxaliplatin resistance in colorectal cancer. Theranostics 12:4386-4398 (2022). PubMed: 35673560
- Gonzales B et al. Type I Phosphatidylinositol-4-Phosphate 5-Kinases a and ? Play a Key Role in Targeting HIV-1 Pr55Gag to the Plasma Membrane. J Virol 94:N/A (2020). PubMed: 32376619
- Ni W et al. SUMOylation is required for PIPK1?-driven keratinocyte migration and growth. FEBS J 286:4709-4720 (2019). PubMed: 31276292
- Wang DG et al. PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism. Cell Rep 27:1991-2001.e5 (2019). PubMed: 31091439
- Peng W et al. Type I? phosphatidylinositol phosphate kinase promotes tumor growth by facilitating Warburg effect in colorectal cancer. EBioMedicine 44:375-386 (2019). PubMed: 31105034
- Durand N et al. The phosphorylation status of PIP5K1C at serine 448 can be predictive for invasive ductal carcinoma of the breast. Oncotarget 9:36358-36370 (2018). PubMed: 30555634
- Jafari N et al. p70S6K1 (S6K1)-mediated Phosphorylation Regulates Phosphatidylinositol 4-Phosphate 5-Kinase Type I ? Degradation and Cell Invasion. J Biol Chem 291:25729-25741 (2016). PubMed: 27780861
- Tojima T et al. Steering neuronal growth cones by shifting the imbalance between exocytosis and endocytosis. J Neurosci 34:7165-78 (2014). PubMed: 24849351
- Chen C et al. Integrin a9ß1 in airway smooth muscle suppresses exaggerated airway narrowing. J Clin Invest 122:2916-27 (2012). PubMed: 22772469