为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Neuroscience Neurotransmission Secretory Vesicles Other

Anti-PIP2抗体(ab2335)

  • Datasheet
  • SDS
Submit a review Q&A (6)References (10)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Key features and details

  • Mouse monoclonal to PIP2
  • Isotype: IgG2b

你可能会感兴趣

一抗
Product image
HRP Anti-Protein A antibody (ab18596)
二抗
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
一抗
Product image
Anti-SLUG antibody [mAbcam51772] (ab51772)

查看更多相关产品

概述

  • 产品名称

    Anti-PIP2抗体
    参阅全部 PIP2 一抗
  • 描述

    小鼠单克隆抗体to PIP2
  • 宿主

    Mouse
  • 特异性

    This antibody cross reacts with Phosphatidic acid (<5.0%).
  • 免疫原

    Phosphatidylinositol-4,5-biphosphate (PIP2) from bovine spinal cord

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituents: PBS, 10% Fetal calf serum
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 同种型

    IgG2b
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Other
    • Signal Transduction
    • Second Messenger
    • Lipid Messengers
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

靶标

  • 相关性

    Phosphatidylinositol 4,5-biphosphate (PIP2) is a membrane phospholipid that has been implicated in a variety of cellular processes, including synaptic vesicle recycling and signal transduction pathways. PLCD4 hydrolyzes PIP2 to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3).

实验方案

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (10)

    发表研究结果有使用 ab2335?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2335 被引用在 10 文献中.

    • Suzuki H  et al. Regulation of MC3T3-E1 differentiation by actin cytoskeleton through lipid mediators reflecting the cell differentiation stage. Biochem Biophys Res Commun 514:393-400 (2019). PubMed: 31047639
    • Assil S  et al. Plasmacytoid Dendritic Cells and Infected Cells Form an Interferogenic Synapse Required for Antiviral Responses. Cell Host Microbe 25:730-745.e6 (2019). PubMed: 31003939
    • Roman-Garcia S  et al. Distinct Roles for Bruton's Tyrosine Kinase in B Cell Immune Synapse Formation. Front Immunol 9:2027 (2018). PubMed: 30237801
    • Mkrtschjan MA  et al. Lipid signaling affects primary fibroblast collective migration and anchorage in response to stiffness and microtopography. J Cell Physiol 233:3672-3683 (2018). PubMed: 29034471
    • Wahl S  et al. The Disease Protein Tulp1 Is Essential for Periactive Zone Endocytosis in Photoreceptor Ribbon Synapses. J Neurosci 36:2473-93 (2016). PubMed: 26911694
    • Aoun J  et al. Anoctamin 6 Contributes to Cl- Secretion in Accessory Cholera Enterotoxin (Ace)-stimulated Diarrhea: AN ESSENTIAL ROLE FOR PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE (PIP2) SIGNALING IN CHOLERA. J Biol Chem 291:26816-26836 (2016). PubMed: 27799301
    • Li J  et al. Actin dynamics is rapidly regulated by the PTEN and PIP2 signaling pathways leading to myocyte hypertrophy. Am J Physiol Heart Circ Physiol 307:H1618-25 (2014). PubMed: 25260617
    • Brzeska H  et al. Molecular basis of dynamic relocalization of Dictyostelium myosin IB. J Biol Chem 287:14923-36 (2012). ICC/IF . PubMed: 22367211
    • Goldston AM  et al. Exposure to host ligands correlates with colocalization of Gal/GalNAc lectin subunits in lipid rafts and phosphatidylinositol (4,5)-bisphosphate signaling in Entamoeba histolytica. Eukaryot Cell 11:743-51 (2012). PubMed: 22505337
    • Ehrlich LS  et al. Activation of the inositol (1,4,5)-triphosphate calcium gate receptor is required for HIV-1 Gag release. J Virol 84:6438-51 (2010). PubMed: 20427533

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Question


    I am wondering if ab2335 is the same clone (KT 10) as was developed by Fukami, et al in the 1988 PNAS paper (Vol. 85 pp.9057-61). If not, any information about the development of this specific clone would be much appreciated.
    I am trying to look for PIP2 in Megakaryocytes (hematopoeitic cells) and am looking for a good ab for IF.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 19 2012

    Answer

    Thank you for contacting Abcam

    I have talked to the supplier of this antibody and I am sorry but they do not have any detailed clone information that I can share and cannot confirm that this antibody is the same as the one published in the paper indicated.
    Sorry that I cannot be of more help in this matter, but if there is anything else I can help you with, please elt me know.

    Free RabMab with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    回复于 Nov 19 2012

    Question

    Does this antibody cross-react with PIP3? What protocols were used to test this antibody in ELISA and ICC?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 14 2012

    Answer

    Thank you very much for your call last week, and I'm very sorry for the delay getting back to you.

    I've just heard from the lab, and unfortunately ab2335 has not been tested for cross-reactivity with PIP3. The original clone was produced and tested by an outside lab, and we don't have the full ELISA or ICC protocols at this time.

    I apologize that we don't have more information about this antibody, but if you have further questions or comments, or if there is anything else that we can do for you, please let me know and I'll be happy to help.

    Read More

    Abcam Scientific Support

    回复于 Nov 14 2012

    Question

    What is the cross reactivity of this antibody with other PtdIns?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 30 2007

    Answer

    Thank you for your enquiry. The cross reactivity of this antibody with other PtdIns is stated below. PIP2 100%; Phosphatidic acid <5.0%; PIP 0.1%; Phosphatidyl inositol <0.2%; Phosphatidyl choline <0.2%; Phosphatidyl serine <0.2%; Phosphatidyl ethanolamine <0.2%; Phosphatidyl glycerol <0.2%; Cardiolipin <0.2%; Cholesterol <0.2%; Diacylglycerol <0.2%

    Read More

    Abcam Scientific Support

    回复于 Mar 30 2007

    Question

    Is ab2335 clone 2C11?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 26 2005

    Answer

    Thank you for your enquiry. No ab2335 is not clone 2C11. Please do not hesitate to contact me should you require further assistance.

    Read More

    Abcam Scientific Support

    回复于 Sep 27 2005

    Question

    Has ab2335 been tested yet for immunocytochemistry?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 02 2004

    Answer

    To our knowledge, this antibody has yet to be tested in ICC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 Nov 02 2004

    Question

    According to your datasheet, ab2335's tested applications are ELISA and radioimmunoassay. Is there a published ELISA potocol using ab2335? If so, what is the reference? Also, is there information on the cross reactivity to other phosphatidylinositols? regards,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 01 2004

    Answer

    Thank you for your enquiry, Here are two publications citing the use of this antibody: RIA: Fukami, K., Takenawa, T. (1989) Quantitative Changes in Polysphophoinositides 1,2-diacyglycerol and Inositol 1,4,5-Trisphosphate by Platelet-Derived Growth Factor and Prostaglandin F2a. The Journal of Biological Chemistry 264(25):1485-1489. ELISA: Imai, K., et al. (1999) The Pleckstrin Homology Domain of the Wiskott-Aldrich Syndrome Protein Is Involved in the Organization of Actin Cytoskeleton. Clinical Immunology 92(2):128-137. We do not have any information regarding the cross reactivity to other phosphatidylinositols, my apologies for the inconvenience,

    Read More

    Abcam Scientific Support

    回复于 Nov 02 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    ?ICP?14046373?-1

    ????? 31011502007413?

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有