Anti-PI 3 Kinase p110 delta抗体(ab1678)
Key features and details
- Rabbit polyclonal to PI 3 Kinase p110 delta
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PI 3 Kinase p110 delta抗体
参阅全部 PI 3 Kinase p110 delta 一抗 -
描述
兔多克隆抗体to PI 3 Kinase p110 delta -
宿主
Rabbit -
特异性
Binds to a 110 Kd protein. Not likely to cross react with other isoforms due to sequence dissimilarity. -
Tested Applications & Species
Application Species ICC/IF Human -
免疫原
20 amino acid C-terminal peptide to Phosphoinositide 3 Kinase p110 delta
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
---|---|
ICC/IF |
Human
|
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use a concentration of 1 µg/ml.
|
说明 |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
-
组织特异性
Expressed predominantly in leukocytes. -
通路
Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis. -
序列相似性
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. -
翻译后修饰
Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity. - Information by UniProt
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数据库链接
- Entrez Gene: 5293 Human
- Omim: 602839 Human
- SwissProt: O00329 Human
- Unigene: 518451 Human
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别名
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
- 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
- APDS antibody
see all
图片
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ICC/IF image of ab1678 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1678, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab1678 被引用在 21 文献中.
- Shin YJ et al. PIP4K2A as a negative regulator of PI3K in PTEN-deficient glioblastoma. J Exp Med 216:1120-1134 (2019). PubMed: 30898893
- Mei XL & Zhong S Long noncoding RNA LINC00520 prevents the progression of cutaneous squamous cell carcinoma through the inactivation of the PI3K/Akt signaling pathway by downregulating EGFR. Chin Med J (Engl) 132:454-465 (2019). PubMed: 30707166
- Feng Z et al. CD40L inhibits cell growth of THP-1 cells by suppressing the PI3K/Akt pathway. Onco Targets Ther 12:3011-3017 (2019). PubMed: 31114244
- Romeo V et al. Effects of PI3K Inhibition on Afucosylated Antibody-Driven Fc?RIIIa Events and Phospho-S6 Activity in NK Cells. J Immunol 203:137-147 (2019). PubMed: 31092639
- Xu D et al. Ligustrazine Inhibits Growth, Migration and Invasion of Medulloblastoma Daoy Cells by Up-Regulation of miR-211. Cell Physiol Biochem 49:2012-2021 (2018). PubMed: 30244244