Anti-PI 3 Kinase p110 delta抗体(ab1678)

Reviews (3)Q&A (3)References (21)

Key features and details

  • Rabbit polyclonal to PI 3 Kinase p110 delta
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-PI 3 Kinase p110 delta抗体
    参阅全部 PI 3 Kinase p110 delta 一抗

  • 描述

    兔多克隆抗体to PI 3 Kinase p110 delta

  • 宿主

    Rabbit

  • 特异性

    Binds to a 110 Kd protein. Not likely to cross react with other isoforms due to sequence dissimilarity.

  • 经测试应用

    适用于: ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    20 amino acid C-terminal peptide to Phosphoinositide 3 Kinase p110 delta

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab1678 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 组织特异性

    Expressed predominantly in leukocytes.

  • 通路

    Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis.

  • 序列相似性

    Belongs to the PI3/PI4-kinase family.
    Contains 1 PI3K/PI4K domain.

  • 翻译后修饰

    Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
  • Information by UniProt

  • 数据库链接

    • 别名

      • 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
      • 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
      • APDS antibody
      • GRB1 antibody
      • IMD14 antibody
      • p110d antibody
      • p110delta antibody
      • p110dp85a antibody
      • p85-ALPHA antibody
      • Phosphatidylinositol 3 kinase catalytic delta polypeptide antibody
      • Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit delta isoform antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit delta antibody
      • Phosphatidylinositol 4,5 bisphosphate 3 kinase, catalytic subunit delta antibody
      • Phosphatidylinositol 45 bisphosphate 3 kinase catalytic subunit delta isoform antibody
      • Phosphatidylinositol-4 antibody
      • Phosphoinositide 3 kinase B antibody
      • Phosphoinositide 3 kinase C antibody
      • Phosphoinositide 3 kinase catalytic delta polypeptide antibody
      • Phosphoinositide 3 kinase, catalytic, delta polypeptide variant p37delta antibody
      • PI3 kinase p110 subunit delta antibody
      • PI3-kinase subunit delta antibody
      • PI3K antibody
      • PI3K-delta antibody
      • PI3Kdelta antibody
      • Pik3cd antibody
      • PIK3R1 antibody
      • PK3CD antibody
      • PK3CD_HUMAN antibody
      • PtdIns 3 kinase p110 antibody
      • PtdIns 3 kinase subunit p110 delta antibody
      • PtdIns-3-kinase subunit delta antibody
      • PtdIns-3-kinase subunit p110-delta antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody (ab1678)
      Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody (ab1678)

      ICC/IF image of ab1678 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1678, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

    文献 (21)

    发表研究结果有使用 ab1678？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab1678 被引用在 21 文献中.

    • Shin YJ  et al. PIP4K2A as a negative regulator of PI3K in PTEN-deficient glioblastoma. J Exp Med 216:1120-1134 (2019). PubMed: 30898893
    • Mei XL & Zhong S Long noncoding RNA LINC00520 prevents the progression of cutaneous squamous cell carcinoma through the inactivation of the PI3K/Akt signaling pathway by downregulating EGFR. Chin Med J (Engl) 132:454-465 (2019). PubMed: 30707166
    • Feng Z  et al. CD40L inhibits cell growth of THP-1 cells by suppressing the PI3K/Akt pathway. Onco Targets Ther 12:3011-3017 (2019). PubMed: 31114244
    • Romeo V  et al. Effects of PI3K Inhibition on Afucosylated Antibody-Driven Fc?RIIIa Events and Phospho-S6 Activity in NK Cells. J Immunol 203:137-147 (2019). PubMed: 31092639
    • Xu D  et al. Ligustrazine Inhibits Growth, Migration and Invasion of Medulloblastoma Daoy Cells by Up-Regulation of miR-211. Cell Physiol Biochem 49:2012-2021 (2018). PubMed: 30244244
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-PI 3 Kinase p110 delta antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (K652 cells)
    Loading amount
    5000 cells
    Specification
    K652 cells
    Treatment
    DMSO or 1 uM CAL-101 for 24 hours
    Gel Running Conditions
    Reduced Denaturing (4-20% Bis-Tris)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 21 2013

    Question

    Hello,

    We have a quality complaint for item ab55593. Please check attached file for detail.

    We request money back.

    Thanks,


    Item number: ab55593

    Order number: 1049169



    General Information

    Antibody storage conditions？





    -20









    Description of the problem (high background, wrong band size, more bands, no band etc.)





    no band









    Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)





    Human/Cell extract









    Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)





    RIPA buffer containing protease and phosphatase inhibitor cocktails (Roche Diagnostics) and also heating sample







    Amount of protein loaded





    60, 80μg of total protein extracts









    Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)





    10% SDS-PAGE gels









    Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)





    nitrocellulose membranes (Milliopore) 90 min

    The membranes were blocked with 5% milk powder, washed with TBST







    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)





    Primeary antibody purchased from Abcom named ab55593

    Primary antibody is diluted with 4% BSA. And the membranes were incubated overnight at 4℃. The membranes were washed with TBST three times.







    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)





    peroxidase-conjugated anti-mouse IgG (Sigma) as a secondary antibody

    Incubation for 2 hours was at room temperature.

    Also washed three times by TBST.







    Detection method (ECL, ECLPlus etc.)





    Western Blotting luminal reagent (Santa cruz)







    Positive and negative controls used (please specify)





    Gapdh as positive control









    Instruction

    Repeat times: 3


    Our western blotting experiment is based on the instruction provided by your company, but we got no band. At the same time, we used the protein of 293T cell (published by some paper) to repeat the experiment and we got the same result.

    We have prolonged the time of the incubation and exposure, and also use the Concentration 1:200, but there is still no band of PI3KCB. There is no problem with our technology of western blotting, because we have got the target band of PI3KC3 by antibody from CST.

    Finally, I hope that your company can make up our loss and solve the problems as soon as possible.

    Read More
    Answer

    Thank you for your enquiry regarding ab55593 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.

    I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

    As the datasheet indicates this antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Would you be so kind to confirm the sample types used (species, cells, tissues)?

    We have several alternative antibodies against the same target (PI3 Kinase p110 beta - ab32569, ab97322, ab1678) and I could offer you a free of charge replacement, or a credit note or a full refund as compensation.

    Thank you for your understanding and co-operation in this matter. I look forward to hearing from you and hope to solve this problem as soon as possible.

    Read More

    Question

    I recently purchased your PI3K p110 delta antibody (ab1678). You will be pleased to hear that the antibody appears to work, since recombinant PI3K p110 delta protein appears positive.

    However, if you observe, in the cell sample lanes (3 samples indicated by #number) another band appears around 37kDa.

    Recently a paper was published: p37delta is a new isoform of PI3K p110delta that increases cell proliferation and is over expressed in tumors.
    http://www.nature.com/onc/journal/vaop/ncurrent/full/onc2011492a.html

    It would be really helpful therefore to know exactly the sequence for the immunogen. Your data sheet just says 20 amino acid C-terminal peptide to PI3K p110 delta, which is not enough to know whether it would pick up the p37delta described in the paper above.

    It would be good to know what this other band the antibody picks up is.

    I await hearing your response.

    Read More
    Answer

    Thank you for your data and for bringing this article to our attention.

    The immunogen sequence is

    *************

    Is that included in the p37delta sequence?

    I am glad to see the antibody was specific for the p110 delta isoform. I encourage you to submit your results in an Abreview. There are currently two examples of western blot reviews on the online datasheet.

    https://www.abcam.com/pi-3-kinase-p110-delta-antibody-ab1678-reviews.html

    For information on how to submit a review (for which you will receive credit for a future purchase or gift card), please see the page at this link:

    www.abcam.com/abreviews

    Read More

    Western blot abreview for Anti-PI 3 Kinase p110 delta antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (monocyte, macrophage, whole lung)
    Loading amount
    20 µg
    Specification
    monocyte, macrophage, whole lung
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 03 2009

    Western blot abreview for Anti-PI 3 Kinase p110 delta antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (cancer cells)
    Loading amount
    15 µg
    Specification
    cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 07 2007

    Question

    Mr kamal puri (kpuri@icos.com) Is PI3Kinase antibody (ab1678) a blocking antibody?

    Read More
    Answer

    We have no evidence that PI3Kinase ab1678 is a blocking antibody

    Read More

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