重组Anti-PHPT1抗体[001] - BSA and Azide free (ab276060)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [001] to PHPT1 - BSA and Azide free
- Suitable for: ICC, Flow Cyt (Intra), IHC-P
- Reacts with: Human
概述
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产品名称
Anti-PHPT1抗体[001] - BSA and Azide free
参阅全部 PHPT1 一抗 -
描述
兔单克隆抗体[001] to PHPT1 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: ICC, Flow Cyt (Intra), IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human PHPT1 aa 2-125.
Database link: Q9NRX4-1 -
阳性对照
- IHC-P: Human skeletal muscle tissue. Flow Cyt (Intra): THP1 cells. ICC: HeLa cells.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
001 -
同种型
IgG
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab276060于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
1/20 - 1/100.
|
|
Flow Cyt (Intra) |
1/25 - 1/100.
|
|
IHC-P |
1/2500 - 1/10000.
|
说明 |
---|
ICC
1/20 - 1/100. |
Flow Cyt (Intra)
1/25 - 1/100. |
IHC-P
1/2500 - 1/10000. |
靶标
-
功能
Exhibits phosphohistidine phosphatase activity. -
组织特异性
Expressed abundantly in heart and skeletal muscle. -
序列相似性
Belongs to the janus family. -
细胞定位
Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 29085 Human
- Omim: 610167 Human
- SwissProt: Q9NRX4 Human
- Unigene: 409834 Human
-
别名
- 14 kDa phosphohistidine phosphatase antibody
- CGI 202 antibody
- HSPC141 antibody
see all
图片
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Immunofluorescence staining of Human PHPT1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab276060 at 1/60 dilution at 37ºC 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
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Intracellular flow cytometric analysis of Human PHPT1 expression on THP1 cells. The cells were treated according to manufacturers manuall, stained with ab276060, then a FITC-conjugated secondary antibody (black line) compared with a negative control (grey line). The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Immunochemical staining of human PHPT1 in human skeletal muscle with ab276060 at 1/5000 dilution, formalin-fixed paraffin embedded sections.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab276060 尚未被引用在任何文献中。