Key features and details
- Rabbit polyclonal to PHOX2A
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
参阅全部 PHOX2A 一抗
经测试应用适用于: WBmore details
种属反应性与反应: Rat, Human
预测可用于: Mouse, Rabbit, Horse, Cow, Pig, Macaque monkey, Orangutan
Synthetic peptide corresponding to Mouse PHOX2A aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following whole cell lysates: SHSY5Y; SKNBE; PC12.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab130121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 29 kDa).|
功能May be involved in regulating the specificity of expression of the catecholamine biosynthetic genes. Acts as a transcription activator/factor. Could maintain the noradrenergic phenotype.
疾病相关Defects in PHOX2A are the cause of congenital fibrosis of extraocular muscles type 2 (CFEOM2) [MIM:602078]. CFEOM encompasses several different inherited strabismus syndromes characterized by congenital restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. CFEOM is characterized clinically by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. CFEOM2 may result from the aberrant development of the oculomotor (nIII), trochlear (nIV) and abducens (nVI) cranial nerve nuclei.
序列相似性Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- Aristaless homeobox (Drosophila) fibrosis of extraocular muscles congenital 2 autosomal recessive antibody
- Aristaless homeobox gene homolog (Drosophila) antibody
- Aristaless homeobox homolog antibody
All lanes : Anti-PHOX2A antibody (ab130121) at 1 µg/ml
Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - Negative Control
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 34,36 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 200 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The predicted molecular weight of PHOX2A is 29 kDa (SwissProt), however we expect to observe a banding pattern around 36 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. Please note that HEK293 (lane 4) was used as a negative control for PHOX2A. This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab130121 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.