Anti-Phosphotyrosine抗体[IG2] (ab5592)
![Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (ab5592)](https://www.abcam.cn/ps/datasheet/Images/5/ab5592/ab5592_1.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (ab5592)")
Key features and details
- Mouse monoclonal [IG2] to Phosphotyrosine
- Suitable for: ELISA, ICC/IF, WB, ICC, Flow Cyt, IP
- Reacts with: Species independent
- Isotype: IgG1
概述
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产品名称
Anti-Phosphotyrosine抗体[IG2]
参阅全部 Phosphotyrosine 一抗 -
描述
小鼠单克隆抗体[IG2] to Phosphotyrosine -
宿主
Mouse -
特异性
ab5592 detects phosphotyrosine from most species and tissues. ab5592 will not react with any other phosphorylated amino acids, proteins or nucleotides. -
经测试应用
适用于: ELISA, ICC/IF, WB, ICC, Flow Cyt, IPmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Phosphotyrosine. O-phospho-L-tyrosine and O-phospho-DL-tyramine.
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阳性对照
- HeLa cells.
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
单克隆 -
克隆编号
IG2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab5592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | 1/50 - 1/200. | |
| WB | 1/100 - 1/1000. | |
| ICC | 1/50 - 1/200. | |
| Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IP | Use a concentration of 2 µg/ml. |
靶标
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相关性
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (ab5592)Immunolocalization of phosphotyrosine in HeLa cells using ab5592.
实验方案
数据表及文件
文献 (0)
ab5592 尚未被引用在任何文献中。
