Anti-Phosphotyrosine抗体(ab9319)
Key features and details
- Rabbit polyclonal to Phosphotyrosine
- Suitable for: ELISA, IP, WB, IHC-P, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Phosphotyrosine抗体
参阅全部 Phosphotyrosine 一抗 -
描述
兔多克隆抗体to Phosphotyrosine -
宿主
Rabbit -
特异性
Recognize proteins and peptides phosphorylated on tyrosine residues. No cross-reaction with phosphoserine and threonine. -
经测试应用
适用于: ELISA, IP, WB, IHC-P, IHC-Frmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to keyhole limpet haemocyanin. Phospho-tyrosine KLH conjugates
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阳性对照
- Use Vanadium treated cell culture (30 min) for Western Blotting and Tyrosine-phosphorylated peptide for ELISA. Human breast cancer tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.00
Preservative: 0.01% Sodium azide
Constituent: 0.114% Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Affinity purified with phospho-tyrosine on agarose and competitively eluted with N-acetylated phospho-tyrosine. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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ELISA |
1/1000.
|
|
IP |
Use at an assay dependent concentration. 10 µg/500 µg protein sample.
|
|
WB | (2) |
1/250.
|
IHC-P |
1/50.
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|
IHC-Fr |
1/50.
|
说明 |
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ELISA
1/1000. |
IP
Use at an assay dependent concentration. 10 µg/500 µg protein sample. |
WB
1/250. |
IHC-P
1/50. |
IHC-Fr
1/50. |
靶标
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相关性
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
图片
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All lanes : Anti-Phosphotyrosine antibody (ab9319) at 0.5 µg/ml
Lane 1 : 5 ul of crude protein from of HeLa cell lysate treated with fetal calf serum
Lane 2 : 15 ul of crude protein from HeLa cell lysate treated with fetal calf serum
Lane 3 : 5 ul of crude protein from HeLa cell lysate
Lane 4 : 15 ul of crude protein from HeLa cell lysate
Lysates/proteins at 2 mg/ml per lane. -
Immunohistochemistry analysis of human breast cancer with ab9319
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab9319 被引用在 10 文献中.
- Arthur S et al. Unique Regulation of Coupled NaCl Absorption by Inducible Nitric Oxide in a Spontaneous SAMP1/YitFc Mouse Model of Chronic Intestinal Inflammation. Inflamm Bowel Dis 27:1804-1812 (2021). PubMed: 34019094
- Verheyen T et al. Spatial organization-dependent EphA2 transcriptional responses revealed by ligand nanocalipers. Nucleic Acids Res 48:5777-5787 (2020). PubMed: 32352518
- Ramani K et al. Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation. Am J Pathol 188:640-655 (2018). PubMed: 29305319
- Duan ZZ et al. Protease activated receptor 1 (PAR1) enhances Src-mediated tyrosine phosphorylation of NMDA receptor in intracerebral hemorrhage (ICH). Sci Rep 6:29246 (2016). PubMed: 27385592
- Yosef E et al. Do Src Kinase and Caveolin Interact Directly with Na,K-ATPase? J Biol Chem 291:11736-50 (2016). PubMed: 27022017
- Nakamura S et al. Novel roles for LIX1L in promoting cancer cell proliferation through ROS1-mediated LIX1L phosphorylation. Sci Rep 5:13474 (2015). PubMed: 26310847
- Shaw A et al. Spatial control of membrane receptor function using ligand nanocalipers. Nat Methods 11:841-6 (2014). PubMed: 24997862
- Debreuil J et al. Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum. J Biol Chem 287:19367-76 (2012). WB . PubMed: 22505718
- Zhong Z et al. Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region. J Neurosci 30:15521-34 (2010). IP . PubMed: 21084607
- Moya A et al. Carbonic anhydrase in the scleractinian coral Stylophora pistillata: characterization, localization, and role in biomineralization. J Biol Chem 283:25475-84 (2008). WB . PubMed: 18617510