Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)抗体(ab4828)
Key features and details
- Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783)抗体
参阅全部 Phospholipase C gamma 1/PLC-gamma-1 一抗 -
描述
兔多克隆抗体to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) -
宿主
Rabbit -
特异性
Phosphorylation site-specific antibody selective for the phosphorylated form of the Phospholipase C gamma 1/PLC-gamma-1 containing a phosphate on tyrosine 783. The antibody has been shown to recognize the endogenous form of Phospholipase C gamma 1/PLC-gamma-1 when phosphorylated on tyrosine 783 in a variety of cell types following treatment by a broad range of extracellular stimuli. Applications include NIH 3T3 cells +/- PDGF. The antibody does not react with a site directed mutant (Y783F).
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经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, a wide range of other species
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免疫原
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783). This region is conserved among many species including mouse, rat, cow and frog.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with nonphosphorylated Phospholipase C gamma 1/PLC-gamma-1 enzymes and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using an Phospholipase C gamma 1/PLC-gamma-1 derived peptide that is phosphorylated at tyrosine 783. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| WB | (1) |
1/1000. Predicted molecular weight: 135 kDa.
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| 说明 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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WB
1/1000. Predicted molecular weight: 135 kDa. |
靶标
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功能
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
序列相似性
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
结构域
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
翻译后修饰
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
细胞定位
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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数据库链接
- Entrez Gene: 281987 Cow
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P08487 Cow
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
see all -
别名
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
图片
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody (ab4828)
Extracts prepared from NIH/3T3 (Mouse embryonic fibroblast cell line) cells exposed to PDGF (10 ng/mL) for 10 minutes were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with 1% BSA, followed by incubation with 0.5 µg/mL ab4828 antibody. After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and the signal was detected by chemiluminescence using the Tropix WesternStar detection method and Kodak BioMax ultraclear film. The data show that PDGF is a strong inducer of Phospholipase C gamma 1/PLC-gamma-1 phosphorylation on Y783 in NIH3T3 cells. Extracts prepared from NIH-3T3 cells exposed to PDGF (10 ng/mL) for 10 minutes were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with 1% BSA, followed by incubation with 0.5 µg/mL ab4828 antibody. After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and the signal was detected by chemiluminescence using the Tropix
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) antibody (ab4828)ab5163 at a diluton of 1/1000 staining Phospholipase C gamma 1/PLC-gamma-1 (phospho Y783) in formalin fixed paraffin embedded sections of a) inflammed human gastric epithelium and b) normal human gastric epithelium by immunohistochemistry.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab4828 被引用在 1 文献中.
- Bates RC et al. Activation of Src and release of intracellular calcium by phosphatidic acid during Xenopus laevis fertilization. Dev Biol N/A:N/A (2013). Xenopus laevis . PubMed: 24269904
