重组Anti-Phospholipase C beta 1/PLCB1抗体[EPR19085] (ab182359)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19085] to Phospholipase C beta 1/PLCB1
- Suitable for: IHC-P, WB, IHC-Fr
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Phospholipase C beta 1/PLCB1抗体[EPR19085]
参阅全部 Phospholipase C beta 1/PLCB1 一抗 -
描述
兔单克隆抗体[EPR19085] to Phospholipase C beta 1/PLCB1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IHC-Frmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human, mouse and rat brain lysates; HeLa, HepG2, NIH/3T3, C6 and PC-12 whole cell lysates. IHC-P: Human cerebrum, mouse hippocampus and rat cerebrum tissues. IHC-Fr: Mouse cerebrum tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19085 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab182359于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 150, 100 kDa (predicted molecular weight: 138 kDa).
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IHC-Fr |
1/200.
Perform antigen retrieval with heated citric acid solution (10mM citric acid pH 6.0 + 0.05% Tween-20). |
说明 |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 150, 100 kDa (predicted molecular weight: 138 kDa). |
IHC-Fr
1/200. Perform antigen retrieval with heated citric acid solution (10mM citric acid pH 6.0 + 0.05% Tween-20). |
靶标
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功能
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. -
疾病相关
Epileptic encephalopathy, early infantile, 12 -
序列相似性
Contains 1 C2 domain.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain. -
细胞定位
Nucleus membrane. Cytoplasm. Colocalizes with the adrenergic receptors, ADREN1A and ADREN1B, at the nuclear membrane of cardiac myocytes. - Information by UniProt
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数据库链接
- Entrez Gene: 23236 Human
- Entrez Gene: 18795 Mouse
- Entrez Gene: 24654 Rat
- Omim: 607120 Human
- SwissProt: Q9NQ66 Human
- SwissProt: Q9Z1B3 Mouse
- SwissProt: P10687 Rat
- Unigene: 431173 Human
see all -
别名
- 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase beta 1 antibody
- 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate antibody
- 1-phosphatidylinositol 4 antibody
see all
图片
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All lanes : Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PLCB1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Mouse heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 138 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab182359 observed at 150 kDa. Red - loading control ab8245 observed at 36 kDa.
ab182359 Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] was shown to specifically react with Phospholipase C beta 1/PLCB1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266033 (knockout cell lysate ab257589) was used. Wild-type and Phospholipase C beta 1/PLCB1 knockout samples were subjected to SDS-PAGE. ab182359 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution
Lane 1 : Human brain lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 138 kDa
Observed band size: 100,150 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID: 8429045, 2468162).
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All lanes : Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 138 kDa
Observed band size: 100,150 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 8429045, 2468162).
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All lanes : Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 138 kDa
Observed band size: 100,150 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 8429045, 2468162).
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All lanes : Anti-Phospholipase C beta 1/PLCB1 antibody [EPR19085] (ab182359) at 1/1000 dilution
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 138 kDa
Observed band size: 100,150 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 3minutes; Lane 2 and 3: 10 seconds.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 8429045, 2468162).
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Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on normal Human cerebrum is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on normal Human colon. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human gastric cancer. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on mouse hippocampus is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on mouse kidney. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on rat cerebrum is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on rat kidney. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded frozen, 0.2% Triton X-100 permeabilized Mouse cerebrum tissue labeling Phospholipase C beta 1/PLCB1 with ab182359 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green).
The result showed cytoplasmic staining on mouse cerebrum.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab182359 被引用在 4 文献中.
- Zhang D et al. Circ_0091579 Serves as a Tumor-Promoting Factor in Hepatocellular Carcinoma Through miR-1225-5p/PLCB1 Axis. Dig Dis Sci N/A:N/A (2021). PubMed: 33559088
- Ouni M et al. MiR-205 is up-regulated in islets of diabetes-susceptible mice and targets the diabetes gene Tcf7l2. Acta Physiol (Oxf) 232:e13693 (2021). PubMed: 34028994
- Vivas O et al. Niemann-Pick Type C Disease Reveals a Link between Lysosomal Cholesterol and PtdIns(4,5)P2 That Regulates Neuronal Excitability. Cell Rep 27:2636-2648.e4 (2019). PubMed: 31141688
- Xu X et al. Inhibition of Methamphetamine Self-Administration and Reinstatement by Central Blockade of Angiotensin II Receptor in Rats. J Pharmacol Exp Ther 369:244-258 (2019). PubMed: 30867225