Phosphatidylcholine Assay试剂盒(Colorimetric/Fluorometric) (ab83377)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.1 nmol/well
概述
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产品名称
Phosphatidylcholine Assay试剂盒(Colorimetric/Fluorometric) -
检测方法
Colorimetric/Fluorometric -
样品类型
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Quantitative -
灵敏度
> 0.1 nmol/well -
范围
0.1 nmol/well - 10 nmol/well -
检测时间
0h 40m -
产品概述
Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (ab83377) is a simple convenient means of measuring phosphatidylcholine in a variety of biological samples. This assay uses an enzyme-coupled reaction to hydrolyze phosphatidylcholine to release choline, which subsequently oxidizes the OxiRed probe in order to generate fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). This assay measures phosphatidylcholine in the range of 0.1 to 10 nmol per sample.
Visit our FAQs page for tips and troubleshooting.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K576 Phosphatidylcholine Colorimetric/Fluorometric Assay Kit. K576-100 is the same size as the 100 test size of ab83377.
Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid.
PC is present in serum at ~0.2-2.5 mM (~50-200 mg/dL).
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer I 1 x 25ml Enzyme Mix IV 1 vial OxiRed Probe 1 x 200µl PC Hydrolysis Enzyme 1 vial Phospholipid Standard 1 vial -
研究领域
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相关性
Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid. -
别名
- PC
相关产品
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Related Products
图片
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Phosphatidycholine levels measured fluorometrically in mouse tissue lysates (mg of extracted protein; background signal subtracted, mean of duplicates; +/- SD).
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Phosphatidycholine levels colorimetrically measured in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
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Phosphatidycholine levels colorimetrically measured in rat biological fluids (background signal subtracted, mean of duplicates; +/- SD).
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Examples of Colorimetric and Fluorometric Phosphatidylcholine standard curves using ab83377.
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab83377 被引用在 23 文献中.
- Yang JW et al. Tissue-level alveolar epithelium model for recapitulating SARS-CoV-2 infection and cellular plasticity. Commun Biol 5:70 (2022). PubMed: 35046486
- Morton RE & Mihna D Apolipoprotein F concentration, activity, and the properties of LDL controlling ApoF activation in hyperlipidemic plasma. J Lipid Res 63:100166 (2022). PubMed: 35016907
- Du C et al. Rheb Promotes Triglyceride Secretion and Ameliorates Diet-Induced Steatosis in the Liver. Front Cell Dev Biol 10:808140 (2022). PubMed: 35372326
- Zappelli E et al. α-Glyceryl-phosphoryl-ethanolamine protects human hippocampal neurons from ageing-induced cellular alterations. Eur J Neurosci 56:4514-4528 (2022). PubMed: 35902984
- Chiang YP et al. Sphingomyelin synthase related protein is a mammalian phosphatidylethanolamine phospholipase C. Biochim Biophys Acta Mol Cell Biol Lipids 1866:159017 (2021). PubMed: 34332077