Key features and details
- Rabbit polyclonal to PHF16
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
经测试应用适用于: WB, IPmore details
预测可用于: Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 773-823 of Human PHF16 (NP_055550.1).
- HeLa, 293T and Jurkat whole cell lysates.
存放说明Shipped at 4°C. Store at +4°C.
存储溶液Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
纯度Immunogen affinity purified
纯化说明ab129495 was affinity purified using an epitope specific to PHF16 immobilized on solid support.
Our Abpromise guarantee covers the use of ab129495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 94 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
功能Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo.
组织特异性Ubiquitously expressed, with highest levels in placenta and uterus.
序列相似性Belongs to the JADE family.
Contains 1 PHD-type zinc finger.
- Information by UniProt
- JADE 3 antibody
- Jade family PHD finger protein 3 antibody
- JADE3 antibody
All lanes : Anti-PHF16 antibody (ab129495) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 94 kDa
Exposure time: 3 minutes
Detection of PHF16 by Western Blot of Immunprecipitate.
ab129495 at 1 µg/ml staining PHF16 in HeLa whole cell lysate immunoprecipitated using ab129495 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Predicted band size : 94 kDa.
Detection: Chemiluminescence with exposure time of 3 minutes.
ab129495 被引用在 1 文献中.
- Pardo M et al. Myst2/Kat7 histone acetyltransferase interaction proteomics reveals tumour-suppressor Niam as a novel binding partner in embryonic stem cells. Sci Rep 7:8157 (2017). PubMed: 28811661