Anti-PHAP1抗体(ab5992)

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Western blot - Anti-PHAP1 antibody (ab5992)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
  • Western blot - Anti-PHAP1 antibody (ab5992)
  • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)

Key features and details

  • Rabbit polyclonal to PHAP1
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-PHAP1抗体
    参阅全部 PHAP1 一抗

  • 描述

    兔多克隆抗体to PHAP1

  • 宿主

    Rabbit

  • 特异性

    This antibody does not cross-react with PHAP12a or PHAPIII.

  • 经测试应用

    适用于: ICC/IF, WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Rat

  • 免疫原

    Synthetic peptide corresponding to Human PHAP1.
    (Peptide available as ab6241)

  • 阳性对照

    • WB: Daudi, HeLa, HepG2, K562 and Raji cell lysates. ICC/IF: Raji cells

  • 常规说明

    Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5992于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 2 µg/ml.
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.
说明
ICC/IF
Use a concentration of 2 µg/ml.
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.

靶标

  • 功能

    Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.

  • 组织特异性

    Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.

  • 序列相似性

    Belongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.

  • 翻译后修饰

    Phosphorylated on serine residues.
    The N-terminus is blocked.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.

  • 细胞定位

    Nucleus. Cytoplasm. Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
  • Information by UniProt

  • 数据库链接

    • 别名

      • acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
      • Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
      • Acidic nuclear phosphoprotein 32 family member A antibody
      • Acidic nuclear phosphoprotein pp32 antibody
      • AN32A_HUMAN antibody
      • ANP32A antibody
      • C15orf1 antibody
      • Cerebellar leucine rich acidic nuclear protein antibody
      • Hepatopoietin Cn antibody
      • HPPCn antibody
      • I1PP2A antibody
      • Inhibitor 1 of protein phosphatase 2A antibody
      • inhibitor-1 of protein phosphatase-2A antibody
      • Lanp antibody
      • Leucine rich acidic nuclear protein antibody
      • Leucine-rich acidic nuclear protein antibody
      • MAPM antibody
      • Mapmodulin antibody
      • MGC119787 antibody
      • MGC150373 antibody
      • PHAPI antibody
      • Potent heat stable protein phosphatase 2A inhibitor I1PP2A antibody
      • Potent heat-stable protein phosphatase 2A inhibitor I1PP2A antibody
      • PP32 antibody
      • Putative HLA DR associated protein I antibody
      • Putative HLA-DR-associated protein I antibody
      • Putative human HLA class II associated protein I antibody
      • Putative human HLA class II-associated protein antibody
      see all

    图片

    • Western blot - Anti-PHAP1 antibody (ab5992)
      Western blot - Anti-PHAP1 antibody (ab5992)
      All lanes : Anti-PHAP1 antibody (ab5992) at 2 µg/ml

      Lane 1 : Daudi cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : HepG2 cell lysates
      Lane 4 : K562 cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
    • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
      Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)

      Immunofluorescent analysis of 4% paraformaldehydefixed Raji Cells labeling PHAP I with ab5992 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

    • Western blot - Anti-PHAP1 antibody (ab5992)
      Western blot - Anti-PHAP1 antibody (ab5992)
      Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively. Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively.
    • Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)
      Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)

      Immunocytochemical analysis of Raji cells labeling PHAP1 with ab5992 at 2 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

    文献 (1)

    发表研究结果有使用 ab5992？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab5992 被引用在 1 文献中.

    • de Souza CF  et al. Creatine transporter immunolocalization in aged human and detached retinas. Invest Ophthalmol Vis Sci 53:1936-45 (2012). PubMed: 22410560

    客户评价及客户问答

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    Question

    I wish to buy the anti PHAPI antibody however I cant tell what the difference is between ab5992 and ab5991. Is there any difference?

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    Answer

    Thank you for your patience in awaiting a response from us. Ab5992 and Ab5991 are both against the same protein, PHAP1, but the sequences were derived from two different locations; the intermediate domain (IN) and the C-terminus (CT) of the protein respectively.

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