Anti-PHAP1抗体(ab5992)
Key features and details
- Rabbit polyclonal to PHAP1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PHAP1抗体
参阅全部 PHAP1 一抗 -
描述
兔多克隆抗体to PHAP1 -
宿主
Rabbit -
特异性
This antibody does not cross-react with PHAP12a or PHAPIII. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to Human PHAP1.
(Peptide available asab6241) -
阳性对照
- WB: Daudi, HeLa, HepG2, K562 and Raji cell lysates. ICC/IF: Raji cells
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常规说明
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab5992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 2 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa. |
靶标
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功能
Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression. -
组织特异性
Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala. -
序列相似性
Belongs to the ANP32 family.
Contains 4 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain. -
翻译后修饰
Phosphorylated on serine residues.
The N-terminus is blocked.
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
细胞定位
Nucleus. Cytoplasm. Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 8125 Human
- Entrez Gene: 11737 Mouse
- Entrez Gene: 25379 Rat
- Omim: 600832 Human
- SwissProt: P39687 Human
- SwissProt: O35381 Mouse
- SwissProt: P49911 Rat
- Unigene: 458747 Human
see all -
别名
- acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
- Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
- Acidic nuclear phosphoprotein 32 family member A antibody
see all
图片
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Western blot - Anti-PHAP1 antibody (ab5992)All lanes : Anti-PHAP1 antibody (ab5992) at 2 µg/ml
Lane 1 : Daudi cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : HepG2 cell lysates
Lane 4 : K562 cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution -
Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)Immunofluorescent analysis of 4% paraformaldehydefixed Raji Cells labeling PHAP I with ab5992 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Western blot - Anti-PHAP1 antibody (ab5992)Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2µ g/ml (lane A) and 4µ g/ml (lane B), respectively. Western blot analysis of PHAP I expression in human Raji cell lysate with anti-PHAP I at 2 µg/ml (lane A) and 4 µg/ml (lane B), respectively. -
Immunocytochemistry/ Immunofluorescence - Anti-PHAP1 antibody (ab5992)Immunocytochemical analysis of Raji cells labeling PHAP1 with ab5992 at 2 μg/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
实验方案
数据表及文件
文献 (1)
ab5992 被引用在 1 文献中.
- de Souza CF et al. Creatine transporter immunolocalization in aged human and detached retinas. Invest Ophthalmol Vis Sci 53:1936-45 (2012). PubMed: 22410560
