Key features and details
- Rabbit polyclonal to PHAP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
特异性This antibody cross-reacts with both PHAP I and PHAP III.
经测试应用适用于: WB, IHC-P, ICC/IFmore details
种属反应性与反应: Mouse, Rat, Human
Synthetic peptide (Human).
- Raji whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months.
Preservative: 0.02% Sodium azide
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab5987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects bands of approximately 35 and 32 kDa corresponding to PHAPIII and PHAPI respectively. Two cross reacting bands are also observed below 32 kDa, which may be differentially spliced forms of PHAPI.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
相关性This antibody recognizes all three identified isoforms of human, mouse and rat PHAP, including PHAP I, PHAP I2a, and PHAP III. The PHAP proteins(tumor suppressor putative HLA-DR associated proteins) are important regulators of mitochondrial apoptosis. PHAP facilitates apoptosome-mediated caspase-9 activation to stimulate the mitochondrial apoptosis pathway. In addition, PHAP opposes both Ras- and myc- mediated cell transformation.
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Immunofluorescence of PHAP in Human small intestine tissue with ab5987 at 20 ug/mL.
Western blot analysis of PHAP expression in human Raji cell llysate with anti-PHAP at 1Western blot analysis of PHAP expression in human Raji cell llysate with anti-PHAP at 1 µg/ml. The two bands below PHAP I might be differently spliced isoforms of PHAP.
µg/ml. The two bands below PHAP I might be differently spliced isoforms of PHAP.
ab5987 at 10µg/ml staining PHAP in human small intestine tissue by IHC