Anti-PHAP抗体(ab5987)
Key features and details
- Rabbit polyclonal to PHAP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-PHAP抗体 -
描述
兔多克隆抗体to PHAP -
宿主
Rabbit -
特异性
This antibody cross-reacts with both PHAP I and PHAP III. -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide (Human).
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阳性对照
- Raji whole cell lysate.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab5987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IHC-P | ||
| ICC/IF |
应用说明
IHC-P: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects bands of approximately 35 and 32 kDa corresponding to PHAPIII and PHAPI respectively. Two cross reacting bands are also observed below 32 kDa, which may be differentially spliced forms of PHAPI.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.5 - 2.0 µg/ml. Detects bands of approximately 35 and 32 kDa corresponding to PHAPIII and PHAPI respectively. Two cross reacting bands are also observed below 32 kDa, which may be differentially spliced forms of PHAPI.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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相关性
This antibody recognizes all three identified isoforms of human, mouse and rat PHAP, including PHAP I, PHAP I2a, and PHAP III. The PHAP proteins(tumor suppressor putative HLA-DR associated proteins) are important regulators of mitochondrial apoptosis. PHAP facilitates apoptosome-mediated caspase-9 activation to stimulate the mitochondrial apoptosis pathway. In addition, PHAP opposes both Ras- and myc- mediated cell transformation. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 10541 Human
- Entrez Gene: 8125 Human
- Entrez Gene: 11737 Mouse
- Entrez Gene: 25379 Rat
- Omim: 600832 Human
- SwissProt: P39687 Human
- SwissProt: Q92688 Human
- SwissProt: O35381 Mouse
see all -
别名
- Acidic (leucine-rich) nuclear phosphoprotein 32 family, member B antibody
- Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
- Acidic nuclear phosphoprotein pp32 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-PHAP antibody (ab5987)Immunofluorescence of PHAP in Human small intestine tissue with ab5987 at 20 ug/mL.
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Western blot - Anti-PHAP antibody (ab5987)Western blot analysis of PHAP expression in human Raji cell llysate with anti-PHAP at 1
Western blot analysis of PHAP expression in human Raji cell llysate with anti-PHAP at 1 µg/ml. The two bands below PHAP I might be differently spliced isoforms of PHAP.µ g/ml. The two bands below PHAP I might be differently spliced isoforms of PHAP. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHAP antibody (ab5987)ab5987 at 10µg/ml staining PHAP in human small intestine tissue by IHC
数据表及文件
文献 (0)
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